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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus reuteri
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei574Aminoacyl-adenylateUniRule annotation1
Binding sitei618ATPUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi912ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1
Metal bindingi932ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
ORF Names:LRLP16767_LRLP167_00873Imported
OrganismiLactobacillus reuteriImported
Taxonomic identifieri1598 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 654tRNA-synt_1InterPro annotationAdd BLAST608
Domaini698 – 850Anticodon_1InterPro annotationAdd BLAST153
Domaini906 – 933zf-FPG_IleRSInterPro annotationAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 86"HIGH" regionUniRule annotationAdd BLAST11
Motifi615 – 619"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0U5FG41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGSFFIIKI QELMKGVVTM RVKDTLNLGR TKFPMRGKLP VTEAQREQLW
60 70 80 90 100
EENKVYELRQ KLNEGKPTFV LHDGPPYANG NIHIGHAMNK ISKDFIVRYK
110 120 130 140 150
SMSGYRAPYV PGWDTHGLPI EHQLTKSGYD RKKMSLTEFR DLCREYALKQ
160 170 180 190 200
VDKQRTDFKR LGVSGEWDHP YLTLDKEFEA AQIRVFGEFA KKGLLYQAKK
210 220 230 240 250
PVYWSWSSES ALAEAEVEYH DVVAKTAFFV EQIKDGKGRL DNDTYLVVWT
260 270 280 290 300
TTPWTVPASE AVAVNPKFDY SVVKPANDDR KFVVATDLLE KLAEKLGWED
310 320 330 340 350
YEVVDHLMGQ EFEGMTTQHP YLDRDLLVGL ADYVTADAGT GLVHTAPGYG
360 370 380 390 400
DDDYNFGKKY DLPIFAPIND QGVLTKENGE GFEGVFYQDA DDVSLQKLEE
410 420 430 440 450
NNALLLQEPL QHSYPFDWRT KQPIIFRATD QWFVSIEKMR QNILNALEDV
460 470 480 490 500
KYHPEWGKVR LRNMIKDRGD WVISRQRVWG VPLPIFYAED GTPIMEEETI
510 520 530 540 550
NHVAELFAKY GSNVWFEREA KDLLPEGYTN EHSPNGKFTK ETDIMDVWFD
560 570 580 590 600
SGSSHQGVLA ERDYLTYPAD LYLEGSDQYR GWFNSSLITS VVCSGHAPYK
610 620 630 640 650
EIVSQGFTLD KRGNKMSKSQ GNVIDPNKVV QQMGAEIIRL WVMSADTSAD
660 670 680 690 700
VRVSMGTFQQ ISEAYRKLRN TFRFLLANTS DFNPEENTVS YEKLQSVDKY
710 720 730 740 750
MLVKLNHFLK TMREDFDNYD FLDAYKVLIN FVNNDLSAFY MNIAKDVLYI
760 770 780 790 800
EAENSEVRRS MQTVFYDILL TLVKLLTPIL PHTTEEVWSY LNEPEDFVQL
810 820 830 840 850
TEIPDPRTFA GEEELLSKWD DFMEVRSHVL KSLEEARNAK LIGKSLEAQV
860 870 880 890 900
DLYLTDDQKQ LLDSLNENIQ LLLGVSALHV HSADKAPADA DQYNDGVAVK
910 920 930 940 950
VTTANGETCA RCRMVKEDVG SDPAYPELCA RCAAIVRENF PETVEDGLEE
Length:950
Mass (Da):108,825
Last modified:March 16, 2016 - v1
Checksum:i453ADA877E3370A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LN887744 Genomic DNA Translation: CUR43075.1

Similar proteinsi

Entry informationi

Entry nameiA0A0U5FG41_LACRE
AccessioniPrimary (citable) accession number: A0A0U5FG41
Entry historyiIntegrated into UniProtKB/TrEMBL: March 16, 2016
Last sequence update: March 16, 2016
Last modified: March 28, 2018
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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