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Protein

Pyridoxine 5'-phosphate synthase

Gene

pdxJ

Organism
Yersinia pseudotuberculosis serotype O:1b (strain IP 31758)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.UniRule annotationSAAS annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O.UniRule annotationSAAS annotation

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. D-erythrose-4-phosphate dehydrogenase (epd)
  2. Erythronate-4-phosphate dehydrogenase (pdxB)
  3. Phosphoserine aminotransferase (serC)
  4. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93-amino-2-oxopropyl phosphateUniRule annotation1
Binding sitei203-amino-2-oxopropyl phosphateUniRule annotation1
Active sitei45Proton acceptorUniRule annotation1
Binding sitei471-deoxy-D-xylulose 5-phosphateUniRule annotation1
Binding sitei521-deoxy-D-xylulose 5-phosphateUniRule annotation1
Active sitei72Proton acceptorUniRule annotation1
Binding sitei1021-deoxy-D-xylulose 5-phosphateUniRule annotation1
Sitei153Transition state stabilizerUniRule annotation1
Active sitei193Proton donorUniRule annotation1
Binding sitei1943-amino-2-oxopropyl phosphate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processPyridoxine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00244; UER00313.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine 5'-phosphate synthaseUniRule annotationSAAS annotation (EC:2.6.99.2UniRule annotationSAAS annotation)
Short name:
PNP synthaseUniRule annotation
Gene namesi
Name:pdxJUniRule annotationImported
Ordered Locus Names:YpsIP31758_1139Imported
OrganismiYersinia pseudotuberculosis serotype O:1b (strain IP 31758)Imported
Taxonomic identifieri349747 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000002412 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 121-deoxy-D-xylulose 5-phosphate bindingUniRule annotation2
Regioni215 – 2163-amino-2-oxopropyl phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PNP synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK03474.
OMAiTSNAGWD.

Family and domain databases

CDDicd00003. PNPsynthase. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00279. PdxJ. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR004569. PyrdxlP_synth_PdxJ.
PfamiView protein in Pfam
PF03740. PdxJ. 1 hit.
SUPFAMiSSF63892. SSF63892. 1 hit.
TIGRFAMsiTIGR00559. pdxJ. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0U1QXC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLLLGVNI DHIATLRNAR GTIYPDPVQA AFIAEQAGAD GITVHLREDR
60 70 80 90 100
RHITDRDVRI LRQTIQTRMN LEMAVTDEMV DIACDIKPHF CCLVPEKRQE
110 120 130 140 150
VTTEGGLDVA GQVDKMTLAV GRLADVGILV SLFIDADFRQ IDAAVAAGAP
160 170 180 190 200
YIEIHTGAYA DASTVLERQA ELMRIAKAAT YAAGKGLKVN AGHGLTYHNV
210 220 230 240
QPIAALPEMH ELNIGHAIIG QAVMTGLATA VTDMKVLMRE ARR
Length:243
Mass (Da):26,327
Last modified:February 17, 2016 - v1
Checksum:i0C369688908E1759
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000720 Genomic DNA. Translation: ABS47286.1.
RefSeqiWP_012104785.1. NC_009708.1.

Genome annotation databases

EnsemblBacteriaiABS47286; ABS47286; YpsIP31758_1139.
KEGGiypi:YpsIP31758_1139.

Similar proteinsi

Entry informationi

Entry nameiA0A0U1QXC1_YERP3
AccessioniPrimary (citable) accession number: A0A0U1QXC1
Entry historyiIntegrated into UniProtKB/TrEMBL: February 17, 2016
Last sequence update: February 17, 2016
Last modified: June 7, 2017
This is version 11 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported