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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Yersinia enterocolitica
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
Gene namesi
Name:glnDUniRule annotationImported
ORF Names:ERS008727_00964Imported
OrganismiYersinia enterocoliticaImported
Taxonomic identifieri630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000038698 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini494 – 616HDInterPro annotationAdd BLAST123
Domaini735 – 816ACTInterPro annotationAdd BLAST82
Domaini842 – 916ACTInterPro annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 375UridylyltransferaseUniRule annotationAdd BLAST375
Regioni376 – 734Uridylyl-removingUniRule annotationAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatSAAS annotation

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0T9SU90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRHSPAFL WAAFLSGIHS SGKHMSENHT EHSVSTALTL TIPEQPASPS
60 70 80 90 100
TYLDGELNCP ILKQRLETFQ FWLAEAFNGG TSAETLIAAR SDYIDRLLRR
110 120 130 140 150
LWTFYGFDDV PETALVAVGG YGRGELHPLS DIDVLVLSKQ RLNDKQAQQI
160 170 180 190 200
GQLITLLWDL KLEVGHSVRT LEECLLEGLA DLTVATNMIE SRLICGDVAL
210 220 230 240 250
FLQMQKHIFS DGFWPSPQFF HAKVTEQQER HKRYHGTSYN LEPDIKSSPG
260 270 280 290 300
GLRDIHTLLW VARRHFGATS LGEMVDFGFL TKAERNELIE SQSFLWRIRF
310 320 330 340 350
ALHLVLTRYD NRLLFDRQLS VAQLLQYQGE GNEPVEHMMK DFYRMTRRVS
360 370 380 390 400
ELNNMLLQLF DEAILALDTN EKPRPLDDEF QLRGDLIDLR DENLFVQQPE
410 420 430 440 450
AIMRMFYLMV RNQEIKGIYS TTVRRLRHAR RHLKQPLCQI PEARKLFMAI
460 470 480 490 500
LRHPGAVSRA LLPMHRHSVL WAYMPQWGSI VGQMQFDLFH AYTVDEHTIR
510 520 530 540 550
VLLKLESFAD EKTRPQHPLC VELYPRLPQP ELLLLAALFH DIAKGRGGDH
560 570 580 590 600
SILGAHDVLE FAEQHGLNSR EAQLVAWLVR CHLLMSVTAQ RRDIQDPDVI
610 620 630 640 650
QQFSAEVQSE TRLRYLVSLT VADICATNET LWNSWKQSLL RELYFATEKQ
660 670 680 690 700
LRRGMQNSPD LRDRVRHHRL QALALLRMDN IDEEALHRIW SRCRADYFLR
710 720 730 740 750
HSPNQLAWHA RHLLAHDSSK PLVLVSHQAT RGGTEIFIWS PDRPSLFAAV
760 770 780 790 800
VGELDRRNLS VHDAQIFTNR DGMAMDTFVV LEPDGSPLAQ DRHPIISHAL
810 820 830 840 850
EQAMTQPNYQ HPRVRRLSPK LRHFSVPTET NFLPTHNERR TYLELIALDQ
860 870 880 890 900
PGLLARVGEI FADLGLSLHS ARITTIGERV EDLFVLADKD RRALSVETRR
910
ELAQRLTDTL NPNDKL
Length:916
Mass (Da):105,518
Last modified:February 17, 2016 - v1
Checksum:i5E494451DDA93752
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CQDZ01000004 Genomic DNA Translation: CNK35787.1

Genome annotation databases

EnsemblBacteriaiCNK35787; CNK35787; ERS008727_00964

Similar proteinsi

Entry informationi

Entry nameiA0A0T9SU90_YEREN
AccessioniPrimary (citable) accession number: A0A0T9SU90
Entry historyiIntegrated into UniProtKB/TrEMBL: February 17, 2016
Last sequence update: February 17, 2016
Last modified: January 31, 2018
This is version 17 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health