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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Sinorhizobium fredii USDA 205
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotationSAAS annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotationSAAS annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei419NucleophileUniRule annotation1
Active sitei472Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesisUniRule annotationSAAS annotation, Glycogen metabolismUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
ORF Names:N181_11350Imported
OrganismiSinorhizobium fredii USDA 205Imported
Taxonomic identifieri1230631 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000053340 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 608AamyInterPro annotationAdd BLAST347

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiView protein in InterPro
IPR006048. A-amylase/branching_C.
IPR037439. Branching_enzy.
IPR006407. GlgB.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PANTHERiPTHR43651. PTHR43651. 1 hit.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0T6ZU86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSPPGKDET PAAPGFLPAQ EIEAILSGTH SDPFAVLGVH ACGKSYVARC
60 70 80 90 100
FVPGAATVVA ETLSGNDVGA LERRHDAGFF EGPVALRKAQ PVRYRARNPG
110 120 130 140 150
GEWVVVDPYS FGPVLGPMDD YYIREGSHLR LFDRLGAHPI SHDGAEGFHF
160 170 180 190 200
AVWAPNARRV SVVGSFNDWD GRRHVMRLRA DTGIWEIFIP GVPIGVPYKY
210 220 230 240 250
EILGNDGTLL PLKADPFARR SELRPDTASV TTGEIAQVWD DEAHRRHWDE
260 270 280 290 300
VDPRRQPISI YEVHASSWQR RDNGDMLSWD ELAGRLIPYC VDMGFTHIEF
310 320 330 340 350
LPISEFPYDP SWGYQTTGLY SPTARFGEPE GFARFVNGCH KAGIGVILDW
360 370 380 390 400
VPAHFPTDEH GLRWFDGTAL YEHEDPRQGF HPDWNTAIYN FGREEVAAYL
410 420 430 440 450
VNNALYWAEK FHVDGLRVDA VASMLYLDYS RQHGEWVPNE YGGNENLEAV
460 470 480 490 500
RFLQTMNSRL YGIHQGVLTI AEESTSWPKV SHPVHTGGLG FGFKWNMGFM
510 520 530 540 550
HDTLQYLSRE PVHRKFHHND MTFGLIYAFS ENFVLPLSHD EVVHGKGSLI
560 570 580 590 600
AKMAGDDWQK FANLRAYYAF MWGYPGKKLL FMGQEFAQWR EWSEERQLDW
610 620 630 640 650
NLLEYNLHEG MRRLVRDLNG TYRAKPALHA RDCEGEGFEW LIADDRDNSV
660 670 680 690 700
FAWLRKAPGE KLVAVVTNFT PVYREDYDVP LPVAGRWKEI LNTDAEIYGG
710 720 730
SGKGNGGAVQ AEKRSNGRTM ATITLPPLAT LMLEQD
Length:736
Mass (Da):83,222
Last modified:February 17, 2016 - v1
Checksum:iCECA98F59F6EB0CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AUTC01000045 Genomic DNA. Translation: KSV90612.1.
RefSeqiWP_037394213.1. NZ_AUTC01000045.1.

Genome annotation databases

EnsemblBacteriaiKSV90612; KSV90612; N181_11350.
PATRICifig|1230631.3.peg.1515.

Similar proteinsi

Entry informationi

Entry nameiA0A0T6ZU86_RHIFR
AccessioniPrimary (citable) accession number: A0A0T6ZU86
Entry historyiIntegrated into UniProtKB/TrEMBL: February 17, 2016
Last sequence update: February 17, 2016
Last modified: February 28, 2018
This is version 16 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported