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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Dehalococcoidia bacterium SM23_28_2
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (mtnP)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12PhosphateUniRule annotation1
Sitei167Important for substrate specificityUniRule annotation1
Binding sitei185Substrate; via amide nitrogenUniRule annotation1
Binding sitei186PhosphateUniRule annotation1
Sitei222Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotation, Transferase
Biological processPurine salvageUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
ORF Names:AMJ77_00300Imported
OrganismiDehalococcoidia bacterium SM23_28_2Imported
Taxonomic identifieri1703396 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidia
Proteomesi
  • UP000054036 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 240PNP_UDP_1InterPro annotationAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Phosphate bindingUniRule annotation2
Regioni209 – 211Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Family and domain databases

HAMAPiMF_01963. MTAP. 1 hit.
InterProiView protein in InterPro
IPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR035994. Nucleoside_phosphorylase_sf.
PANTHERiPTHR42679. PTHR42679. 1 hit.
PfamiView protein in Pfam
PF01048. PNP_UDP_1. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0S8EJ20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEARVAIIG GSGFYQMGLT DMEEVRIERT PFGPPSDAIV IGTLDGVRVA
60 70 80 90 100
FLPRHGRGHR ILPSELPSQA NVYALKTLGV EFIIAVCAVG SLRREIEPRQ
110 120 130 140 150
LAVPDQLIDR TKGRPSTFFG QGLTAHIAFA DPFCPVLRDA LWRSAQEAGG
160 170 180 190 200
TAHNGGTLLV MEGPAFSTRA ESNLYRAWGA DLIVMTALPE AKLAREAEIC
210 220 230 240 250
YATLACVTDY DCWYEGQESV TAEMILSNLT KTVEAAQRTV RLALSGLPAR
260 270 280 290
RDCLCASALQ DALVTPLDMV PEETKRNLAP IIGRYVTSES TGS
Length:293
Mass (Da):31,706
Last modified:February 17, 2016 - v1
Checksum:i0ED06A1C693DA8BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LJUA01000002 Genomic DNA. Translation: KPK48270.1.

Genome annotation databases

PATRICifig|1703396.3.peg.62.

Similar proteinsi

Entry informationi

Entry nameiA0A0S8EJ20_9CHLR
AccessioniPrimary (citable) accession number: A0A0S8EJ20
Entry historyiIntegrated into UniProtKB/TrEMBL: February 17, 2016
Last sequence update: February 17, 2016
Last modified: October 25, 2017
This is version 12 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported