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Protein

Probable phosphatidylethanolamine transferase Mcr-1

Gene

mcr1

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Phosphoethanolamine modification of lipid A gives polymyxin resistance (PubMed:26603172).1 Publication
Confers resistance to polymyxin-type antibiotics; expression of the Mcr-1 protein in E.coli increases colistin and polymyxin B minimal inhibitory concentration (MIC) from 0.5 mg/ml to 2.0 mg/ml. The pHNSHP45 plasmid can transfer efficiently (0.1 to 0.001) to other E.coli strains by conjugation and increases polymxin MIC by 8- to 16-fold; it may not require selective pressure to be maintained in the cell. When transformed into K.pneumoniae or P.aeruginosa it also increases polymxin MIC 8- to 16-fold. In a murine (BALB/c mice) thigh infection study using an mcr1-encoding plasmid isolated from a human patient, the plasmid confers in vivo protection against colistin (PubMed:26603172).1 Publication

Miscellaneous

This is the first (as of November 2015) reported plasmid-mediated resistance to polymyxin antibiotics; although it does not confer a high MIC it protects against colistin treatment in an infection model. Colistin is one of the last-resort antibiotics available for multidrug-resistant Gram-negative bacteria such as K.pneumoniae, P.aeruginosa and Acinetobacter. First identified from a pig farm in Shanghai, China in July 2013, retrospective screening detects the gene in (slowly) increasing proportions between 2011 and 2015 from pigs and chickens, and also in hospitalized patients in China. Colistin is very widely used in veterinary agriculture (PubMed:26603172).1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAntibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphatidylethanolamine transferase Mcr-1 (EC:2.7.-.-)
Alternative name(s):
Polymyxin resistance protein MCR-11 Publication
Gene namesi
Name:mcr1
Synonyms:mcr-11 Publication
ORF Names:APZ14_31440
Encoded oniPlasmid pHNSHP450 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000054864 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicCuratedAdd BLAST14
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Topological domaini36 – 47PeriplasmicCuratedAdd BLAST12
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 73CytoplasmicCurated5
Transmembranei74 – 94HelicalSequence analysisAdd BLAST21
Topological domaini95 – 122PeriplasmicCuratedAdd BLAST28
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 157CytoplasmicCuratedAdd BLAST14
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 541PeriplasmicCuratedAdd BLAST363

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004356241 – 541Probable phosphatidylethanolamine transferase Mcr-1Add BLAST541

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi227 – 230Combined sources4
Turni232 – 234Combined sources3
Beta strandi238 – 245Combined sources8
Helixi250 – 252Combined sources3
Helixi254 – 256Combined sources3
Helixi264 – 267Combined sources4
Beta strandi272 – 276Combined sources5
Helixi285 – 292Combined sources8
Helixi297 – 300Combined sources4
Helixi303 – 308Combined sources6
Helixi312 – 318Combined sources7
Beta strandi322 – 330Combined sources9
Turni333 – 338Combined sources6
Helixi341 – 343Combined sources3
Beta strandi344 – 346Combined sources3
Turni350 – 352Combined sources3
Beta strandi357 – 359Combined sources3
Helixi366 – 370Combined sources5
Helixi373 – 379Combined sources7
Turni380 – 382Combined sources3
Beta strandi385 – 390Combined sources6
Helixi399 – 402Combined sources4
Helixi405 – 407Combined sources3
Helixi419 – 421Combined sources3
Helixi424 – 450Combined sources27
Turni451 – 455Combined sources5
Beta strandi456 – 465Combined sources10
Beta strandi469 – 471Combined sources3
Helixi472 – 474Combined sources3
Beta strandi476 – 478Combined sources3
Turni482 – 484Combined sources3
Helixi487 – 489Combined sources3
Beta strandi494 – 498Combined sources5
Helixi500 – 502Combined sources3
Helixi515 – 517Combined sources3
Helixi518 – 525Combined sources8
Helixi531 – 533Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GOVX-ray2.33A/B200-541[»]
5GRRX-ray1.45A219-541[»]
5K4PX-ray1.32A214-541[»]
5LRMX-ray1.75A219-541[»]
5LRNX-ray1.55A/B219-541[»]
SMRiA0A0R6L508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiView protein in InterPro
IPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core_sf.
IPR012549. EptA-like_N.
IPR000917. Sulfatase_N.
PfamiView protein in Pfam
PF08019. DUF1705. 1 hit.
PF00884. Sulfatase. 1 hit.
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0R6L508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQHTSVWYR RSVSPFVLVA SVAVFLTATA NLTFFDKISQ TYPIADNLGF
60 70 80 90 100
VLTIAVVLFG AMLLITTLLS SYRYVLKPVL ILLLIMGAVT SYFTDTYGTV
110 120 130 140 150
YDTTMLQNAL QTDQAETKDL LNAAFIMRII GLGVLPSLLV AFVKVDYPTW
160 170 180 190 200
GKGLMRRLGL IVASLALILL PVVAFSSHYA SFFRVHKPLR SYVNPIMPIY
210 220 230 240 250
SVGKLASIEY KKASAPKDTI YHAKDAVQAT KPDMRKPRLV VFVVGETARA
260 270 280 290 300
DHVSFNGYER DTFPQLAKID GVTNFSNVTS CGTSTAYSVP CMFSYLGADE
310 320 330 340 350
YDVDTAKYQE NVLDTLDRLG VSILWRDNNS DSKGVMDKLP KAQFADYKSA
360 370 380 390 400
TNNAICNTNP YNECRDVGML VGLDDFVAAN NGKDMLIMLH QMGNHGPAYF
410 420 430 440 450
KRYDEKFAKF TPVCEGNELA KCEHQSLINA YDNALLATDD FIAQSIQWLQ
460 470 480 490 500
THSNAYDVSM LYVSDHGESL GENGVYLHGM PNAFAPKEQR SVPAFFWTDK
510 520 530 540
QTGITPMATD TVLTHDAITP TLLKLFDVTA DKVKDRTAFI R
Length:541
Mass (Da):60,124
Last modified:February 17, 2016 - v1
Checksum:i2418130C94864FBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KP347127 Genomic DNA. Translation: AKF16168.1.
LMBK01000308 Genomic DNA. Translation: KST31405.1.
RefSeqiWP_049589868.1. NZ_NQCP01000078.1.

Genome annotation databases

EnsemblBacteriaiKST31405; KST31405; APZ14_31440.

Similar proteinsi

Entry informationi

Entry nameiMCR1_ECOLX
AccessioniPrimary (citable) accession number: A0A0R6L508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 16, 2016
Last sequence update: February 17, 2016
Last modified: November 22, 2017
This is version 15 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families