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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Sphingomonas sp. Root720
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase
LigandMagnesiumUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
ORF Names:ASE22_23125Imported
OrganismiSphingomonas sp. Root720Imported
Taxonomic identifieri1736595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
Proteomesi
  • UP000051570 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini731 – 807ACTInterPro annotationAdd BLAST77
Domaini842 – 920ACTInterPro annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 374UridylyltransferaseUniRule annotationAdd BLAST374

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatSAAS annotation

Family and domain databases

CDDicd00077. HDc. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf. 1 hit.
InterProiView protein in InterPro
IPR002912. ACT_dom.
IPR005105. GlnD_Uridyltrans_N.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
PANTHERiPTHR13734:SF45. PTHR13734:SF45. 2 hits.
PfamiView protein in Pfam
PF03445. DUF294. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiView protein in SMART
SM00471. HDc. 1 hit.
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiView protein in PROSITE
PS51671. ACT. 2 hits.

Sequencei

Sequence statusi: Complete.

A0A0Q8QVW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKFDLLPQ RRAIIDRRMV ADALAGLLPG SSDAAALRAG GTAILKAALD
60 70 80 90 100
TARCEIARRL AEQPTRGSET AAAYAFLTDQ IVRLIYDFTV ERLYPNNNPS
110 120 130 140 150
AAERLTLLAV GGYGRGEMAL HSDVDIAFLT PWKQTSWSEQ VIESMLYSMW
160 170 180 190 200
DLGLKVGHSS RSLDDMIRMA KSDLTIRTAL LEGRFVWGDE ALYDEASTVF
210 220 230 240 250
RRDVVSGTAK TFVIEKLAER NERHRRMGDS RYVVEPNLKE GKGGLRDLHT
260 270 280 290 300
LFWIGKYVYQ VQSVPELVDK GLLTRAELRQ FQKAENFLWA VRCHLHIVAG
310 320 330 340 350
RPEERLTFDV QPEIARRLRY AERPGKSAVE RFMQHYFLTA KRVGDLTAIF
360 370 380 390 400
LAHIDETFAT RGRRFGLPTL RRKPTKLEGF VLDRGRLAIP TDDFFAQDPV
410 420 430 440 450
RLLQMFAIAD RHGLEIHPLA MRAAGRDARL IDNGVRRDPR ANALFMDVLT
460 470 480 490 500
SHNTPETVLR WMNEAGVFGR FVPDFGRVVA QMQFDMYHHY TVDEHSIRAI
510 520 530 540 550
GLLSQIEKGA LKDDHPLSSK IMPGLVQRRV LYVAVLLHDI AKGRGGDHSV
560 570 580 590 600
LGAEVAEKLC PRFGLTPAET ETVSWLVRYH LVMSDTAFKR DLSDFKTILD
610 620 630 640 650
FAERVQSPER LRLLFCLTIV DIRAVGPGVW NSWKRQLLGD LYVAAEEVLR
660 670 680 690 700
LGHKQTGRRE RISAKQAQLR ERLGWDDDRF GRYVARFPEA YWVAESVDIL
710 720 730 740 750
EGNARLIEAA DAGDRPLSIT ASPDPDRGAT LVSIYAGDHP GLFYRIAGAI
760 770 780 790 800
HLAGASVIDA RIHTTRDGMA IDNLLVQDPF GQPFEGDDRM KRLSIAIEDA
810 820 830 840 850
LANRSRMADK LLAKPLARPR ADAFSIEPAA LIDNRASNRY TVIEVNARDR
860 870 880 890 900
PALLYALAHA LFQAKVMIHS AHIATYGERA VDVFYVADLT GDKITSSARL
910 920
KTLERLVVDA AAGAPARKAA
Length:920
Mass (Da):103,114
Last modified:January 20, 2016 - v1
Checksum:iBA04718FAD4F44A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LMID01000015 Genomic DNA. Translation: KRB88321.1.
RefSeqiWP_056366212.1. NZ_LMID01000015.1.

Genome annotation databases

EnsemblBacteriaiKRB88321; KRB88321; ASE22_23125.

Similar proteinsi

Entry informationi

Entry nameiA0A0Q8QVW5_9SPHN
AccessioniPrimary (citable) accession number: A0A0Q8QVW5
Entry historyiIntegrated into UniProtKB/TrEMBL: January 20, 2016
Last sequence update: January 20, 2016
Last modified: November 22, 2017
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported