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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Thermus aquaticus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei266NucleophileUniRule annotation1
Active sitei349UniRule annotation1
Active sitei351UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171
UPA00070; UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chainUniRule annotation
Gene namesi
Name:carAUniRule annotationImported
ORF Names:BVI061214_00859Imported
OrganismiThermus aquaticusImported
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000037685 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini191 – 376Glutamine amidotransferase type-1InterPro annotationAdd BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190CPSaseUniRule annotationAdd BLAST190

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili146 – 166Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the CarA family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis, Glutamine amidotransferaseUniRule annotation

Family and domain databases

CDDicd01744 GATase1_CPSase, 1 hit
Gene3Di3.40.50.8801 hit
3.50.30.201 hit
HAMAPiMF_01209 CPSase_S_chain, 1 hit
InterProiView protein in InterPro
IPR006274 CarbamoylP_synth_ssu
IPR002474 CarbamoylP_synth_ssu_N
IPR036480 CarbP_synth_ssu_N_sf
IPR029062 Class_I_gatase-like
IPR035686 CPSase_GATase1
IPR017926 GATASE
PfamiView protein in Pfam
PF00988 CPSase_sm_chain, 1 hit
PF00117 GATase, 1 hit
SMARTiView protein in SMART
SM01097 CPSase_sm_chain, 1 hit
SUPFAMiSSF52021 SSF52021, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01368 CPSaseIIsmall, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0M9AFI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGMKERAVL VLEDGTVYHG YAFGATGKTV GEVVFNTAQT GYQEIMTDPS
60 70 80 90 100
YHGQIVVMTY PHQGNYGVNV YDMQSNRPWV KGFVAKEFSR IASNPRAQQT
110 120 130 140 150
LGEFMAFYGV VGIEGIDTRA LVRKIREGGV LKGAIAHASL YGSPDHAFTE
160 170 180 190 200
EELKALQEEA RAWTDIDGRD MTPEVSTPLP YAWPTLKSGR RIVVMDFGIK
210 220 230 240 250
HAIVENLAQM GFEILVVPGK TPASQIMALE PHGLLISNGP GDPAMPRYAH
260 270 280 290 300
ETIWKLMGLL PTFGICLGHQ LLALAAGGRT YKMKFGHRGA NHPVKNLQTG
310 320 330 340 350
KIEITSQNHG YAVDIDSLKG FRPTHINLND GTLEGMAHAR YPVFSVQYHP
360 370 380 390
EAAPGPHDAL YLFRRFLEEV EAFHGATGLP VEKQRADQHG I
Length:391
Mass (Da):42,978
Last modified:December 9, 2015 - v1
Checksum:i2F1B4EEC5DAB36FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LHCI01000106 Genomic DNA Translation: KOX89681.1
RefSeqiWP_053767456.1, NZ_LHCI01000106.1

Genome annotation databases

EnsemblBacteriaiKOX89681; KOX89681; BVI061214_00859
PATRICifig|271.14.peg.935

Similar proteinsi

Entry informationi

Entry nameiA0A0M9AFI4_THEAQ
AccessioniPrimary (citable) accession number: A0A0M9AFI4
Entry historyiIntegrated into UniProtKB/TrEMBL: December 9, 2015
Last sequence update: December 9, 2015
Last modified: December 20, 2017
This is version 16 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported