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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Thermus aquaticus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
ORF Names:BVI061214_01700Imported
OrganismiThermus aquaticusImported
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000037685 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 328ATP-graspInterPro annotationAdd BLAST196
Domaini674 – 866ATP-graspInterPro annotationAdd BLAST193

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 409Carboxyphosphate synthetic domainUniRule annotationAdd BLAST409
Regioni934 – 1030Allosteric domainUniRule annotationAdd BLAST97

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotation

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
HAMAPiMF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR036897. CarbamoylP_synth_lsu_oligo_sf.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR016185. PreATP-grasp_dom_sf.
PfamiView protein in Pfam
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PRINTSiPR00098. CPSASE.
SMARTiView protein in SMART
SM01096. CPSase_L_D3. 1 hit.
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 2 hits.

Sequencei

Sequence statusi: Complete.

A0A0M9AEQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPRRDLRKI LIIGSGPITI GQAAEFDYSG TQAVKALRGA GYEAILVNSN
60 70 80 90 100
PATIMTDPDL AERTYVEPLT LEALEKVIAK EAPDALLPTL GGQTALNLSM
110 120 130 140 150
ALHEEGILKR YGVELIGANA EAIKKGEDRE AFQKAMRKIG LEVPRGKMVA
160 170 180 190 200
SVEEGLHFAR EVGFPVVVRP SFTLGGTGGG IAKDEGELKE VLARGLALSP
210 220 230 240 250
VHTALVEESV LGWKEFELEV MRDHADTVVI ITSIENVDPM GVHTGDSITV
260 270 280 290 300
APAQTLSDVE YQRMRDAAKA VIREIGVDTG GSNIQFAVDP KTGRQVVIEM
310 320 330 340 350
NPRVSRSSAL ASKATGFPIA KIAALLAVGY RLDELPNDIT RKTPASFEPT
360 370 380 390 400
IDYVVVKIPR FAFEKFQDLP NTLGGLKDEL GTQMKSVGEV MAMGRTFKEA
410 420 430 440 450
LLKALRGLER DVRALAGVKT EALEKKLYPN PDRIYAVLEL LRRGMGVEEL
460 470 480 490 500
HQATRIDPWF LHQMREIVEA EAWLQDHPPR DREAWRFYKG LGLPDARIGE
510 520 530 540 550
LLGKKEAEVR AERKALGVVP VYKTVDTCAA EFEAYTPYHY STYELEDEVW
560 570 580 590 600
PSKKPKVVIL GSGPIRIGQG VEFDYATVHA VWALKEAGYE TIMVNSNPET
610 620 630 640 650
VSTDYDPADR LYFEPLTLED VLNLVEHEKP LGVIATLGGQ TPLKLAQGLE
660 670 680 690 700
EAGVRLLGTP FQAIHQAEDR EAFHRLCQRL GIPQPEGRVA KSPEEALELA
710 720 730 740 750
KEVGFPLLAR PSYVLGGRAM RVLRSQEELK RYLEEVYGPL QEKPSILLDR
760 770 780 790 800
YLEGALELDV DALSDGEEVL IAGVMEHVER AGVHSGDSAT VLPPVHLSQE
810 820 830 840 850
ALEKVRAYTR RLALALGVRG LLNVQYAVLG EEVYILEANP RASRTVPFVS
860 870 880 890 900
KAIGVPLAKL AALIAVGKSL KELGVRDLDP VPPYFAVKEV VIPWLKFPGV
910 920 930 940 950
IPVLGPEMRS TGESMGLDED PYLAYYKAQL GAGQRLPLSG RVRFIGEGEG
960 970 980 990 1000
VEALKKAYRE AGFALSEGEY DLLISLIPHP ELRRAVEAGL PFITTEEGAL
1010 1020 1030
WSLKAILRAR EKPLEVRSLQ EWHGVYNRAQ
Length:1,030
Mass (Da):113,172
Last modified:December 9, 2015 - v1
Checksum:i685B738D0B595E87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LHCI01000106 Genomic DNA. Translation: KOX90509.1.
RefSeqiWP_053768059.1. NZ_LHCI01000106.1.

Genome annotation databases

EnsemblBacteriaiKOX90509; KOX90509; BVI061214_01700.
PATRICifig|271.14.peg.1771.

Similar proteinsi

Entry informationi

Entry nameiA0A0M9AEQ8_THEAQ
AccessioniPrimary (citable) accession number: A0A0M9AEQ8
Entry historyiIntegrated into UniProtKB/TrEMBL: December 9, 2015
Last sequence update: December 9, 2015
Last modified: March 28, 2018
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported