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Protein

Bifunctional polymyxin resistance protein ArnA

Gene

arnA

Organism
Escherichia coli
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the oxidative decarboxylation of UDP-glucuronic acid (UDP-GlcUA) to UDP-4-keto-arabinose (UDP-Ara4O) and the addition of a formyl group to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N) to form UDP-L-4-formamido-arabinose (UDP-L-Ara4FN). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.UniRule annotationSAAS annotation

Catalytic activityi

10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose.UniRule annotationSAAS annotation
UDP-alpha-D-glucuronate + NAD+ = UDP-beta-L-threo-pentapyranos-4-ulose + CO2 + NADH.UniRule annotationSAAS annotation

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

Pathwayi: UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (b2255), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (yfbG), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (yfbG), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional UDP-glucuronic acid oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase (BET08_22280), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA)
  2. UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (b2253), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (yfbE), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB), UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB)
  3. Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (b2255), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (yfbG), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (yfbG), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional UDP-glucuronic acid oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase (BET08_22280), Bifunctional polymyxin resistance protein ArnA (arnA), Bifunctional polymyxin resistance protein ArnA (arnA)
This subpathway is part of the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-4-deoxy-4-formamido-beta-L-arabinose from UDP-alpha-D-glucuronate, the pathway UDP-4-deoxy-4-formamido-beta-L-arabinose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei102Transition state stabilizerUniRule annotation1
Active sitei104Proton donor; for formyltransferase activityUniRule annotation1
Binding sitei11410-formyltetrahydrofolateUniRule annotation1
Sitei140Raises pKa of active site HisUniRule annotation1
Binding sitei347NADUniRule annotation1
Binding sitei393UDP-glucuronate; via carbonyl oxygenUniRule annotation1
Binding sitei398UDP-glucuronateUniRule annotation1
Active sitei434Proton acceptor; for decarboxylase activityUniRule annotation1
Binding sitei460UDP-glucuronateUniRule annotation1
Binding sitei492UDP-glucuronateUniRule annotation1
Binding sitei613UDP-glucuronateUniRule annotation1
Active sitei619Proton donor; for decarboxylase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi368 – 369NAD bindingUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotationImported, TransferaseUniRule annotationSAAS annotationImported
Biological processAntibiotic resistanceUniRule annotationSAAS annotation, Lipid A biosynthesisUniRule annotationSAAS annotation, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesisUniRule annotationSAAS annotation
LigandNADUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00032; UER00492.
UPA00032; UER00494.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional polymyxin resistance protein ArnAUniRule annotation
Including the following 2 domains:
UDP-glucuronic acid oxidase, UDP-4-keto-hexauronic acid decarboxylatingUniRule annotation (EC:1.1.1.305UniRule annotation)
Alternative name(s):
UDP-glucuronic acid dehydrogenaseUniRule annotation
UDP-GlcUA decarboxylaseUniRule annotation
ArnADHUniRule annotation
UDP-4-amino-4-deoxy-L-arabinose formyltransferaseUniRule annotation (EC:2.1.2.13UniRule annotation)
Alternative name(s):
UDP-L-Ara4N formyltransferaseUniRule annotation
ArnAFTUniRule annotation
Gene namesi
Name:arnAUniRule annotationImported
ORF Names:ERS085366_01123Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000045242 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homohexamer, formed by a dimer of trimers.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 175Formyl_trans_NInterPro annotationAdd BLAST123
Domaini202 – 294Formyl_trans_CInterPro annotationAdd BLAST93
Domaini318 – 566EpimeraseInterPro annotationAdd BLAST249

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 304Formyltransferase ArnAFTUniRule annotationAdd BLAST304
Regioni86 – 8810-formyltetrahydrofolate bindingUniRule annotation3
Regioni136 – 14010-formyltetrahydrofolate bindingUniRule annotation5
Regioni314 – 660Dehydrogenase ArnADHUniRule annotationAdd BLAST347
Regioni432 – 433UDP-glucuronate bindingUniRule annotation2
Regioni526 – 535UDP-glucuronate bindingUniRule annotation10

Sequence similaritiesi

In the C-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the Fmt family. UDP-L-Ara4N formyltransferase subfamily.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_01166. ArnA. 1 hit.
InterProiView protein in InterPro
IPR021168. Bifun_polymyxin_resist_ArnA.
IPR001509. Epimerase_deHydtase.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF01370. Epimerase. 1 hit.
PF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PIRSFiPIRSF036506. Bifun_polymyxin_resist_ArnA. 1 hit.
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF51735. SSF51735. 1 hit.
SSF53328. SSF53328. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0K3NPP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTVVFAYHD MGCLGIEALL AAGYEISAIF THTDNPGEKA FYGSVARLAA
60 70 80 90 100
ERGIPVYAPD NVNHPLWVER IAQLSPDVIF SFYYRHLICD EILQLAPAGA
110 120 130 140 150
FNLHGSLLPK YRGRAPLNWV LVNGETETGV TLHRMVKRAD AGAIVAQLRV
160 170 180 190 200
AIAPDDIAIT LHHKLCHAAR QLLEQTLPAI KHGNILEIAQ RENEATCFGR
210 220 230 240 250
RTPDDSFLEW HKLASVLHNM VRAVADPWPG AFSYVGNQKF TVWSSRVYSH
260 270 280 290 300
APAAQPGSVI SVAPLLIACG DGALEIVTGQ AGDGITMQGS QLAQTLGLVQ
310 320 330 340 350
GSRLNSQPAC TARRRTRVLI LGVNGFIGNH LTERLLREDH YEVYGLDIGS
360 370 380 390 400
DAIIRFLNHP HFHFVEGDIS IHSEWIEYHV KKCDVVLPLV AIATPIEYTR
410 420 430 440 450
NPLRVFELDF EENLRIIRYC VKYRKRIIFP STSEVYGMCS DKYFDEDHSN
460 470 480 490 500
LIVGPVNKPR WIYSVSKQLL DRVIWAYGEK EGLQFTLFRP FNWMGPRLDN
510 520 530 540 550
LNAARIGSSR AITQLILNLV EGSPIKLIDG GKQKRCFTDI RDGVEALYRI
560 570 580 590 600
IENAGNRCDG EIINIGNPDN EASIEELGEM LLASFEKHPL RHHFPPFAGF
610 620 630 640 650
RVVESSSYYG KGYQDVEHRK PSIRNARRCL DWEPKIDMQE TIDETLDFFL
660
RTVELTDKPS
Length:660
Mass (Da):74,231
Last modified:November 11, 2015 - v1
Checksum:iBB763D24C6BD5AA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CYAV01000008 Genomic DNA. Translation: CTS45431.1.
RefSeqiWP_054494929.1. NZ_CYAV01000008.1.

Genome annotation databases

EnsemblBacteriaiCTS45431; CTS45431; ERS085366_01123.

Similar proteinsi

Entry informationi

Entry nameiA0A0K3NPP2_ECOLX
AccessioniPrimary (citable) accession number: A0A0K3NPP2
Entry historyiIntegrated into UniProtKB/TrEMBL: November 11, 2015
Last sequence update: November 11, 2015
Last modified: June 7, 2017
This is version 15 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported, Multifunctional enzymeUniRule annotationSAAS annotation