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Protein

Protein mom-5

Gene

mom-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for Wnt proteins (PubMed:16930586, PubMed:22022276, PubMed:23295860, PubMed:24401370, PubMed:26292279). Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of gsk-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (Probable). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as pkc seems to be required for Wnt-mediated inactivation of gsk-3 kinase (Probable). Both pathways seem to involve interactions with G-proteins (Probable). Required in embryonic development for the correct positioning and orientation of the mitotic spindles and division planes in blastomere cells (PubMed:9288749, PubMed:9288750, PubMed:20126385). During early embryonic cell divisions, directs the asymmetric positioning of transcription factors such as pop-1 and dsh-2 in daughter cells in order to determine cell fate specification (PubMed:12810601, PubMed:15620652, PubMed:15990090). Acts redundantly with other Wnt receptors such as lin-17 to control vulval precursor cell specification and also the polarity of different cell types including distal tip cells, seam cells, AVG interneurons and P-cells and their descendants (PubMed:16930586, PubMed:20126385, PubMed:22022276, PubMed:23295860, PubMed:24401370, PubMed:26292279). Plays a role in the migration of cell types including distal tip cells and the QR neuroblast descendants, QR.p and QR.pa during larval development (PubMed:20126385, PubMed:25373777, PubMed:26292279). Negatively regulates the unc-6/Netrin receptors unc-5 and unc-40 to control distal tip cell polarity and migration (PubMed:26292279). Acts through ced-5/DOCK180 and ced-10/Rac to control both distal tip cell migration and the phagocytic clearance of apoptotic cell corpses (PubMed:20126385). Furthermore, it is also required for the migration and axon guidance of the different neuronal cell types including CAN, ALM, HSN and the two mechanosensory neurons AVM and PVM (PubMed:16109397, PubMed:16516839, PubMed:25917219). Mediates Wnt receptor cfz-2 in directing ALM migration, but may also act redundantly with the Wnt receptors cfz-2 and mig-1 to direct the migration of other neuronal cell types including CAN and HSN (PubMed:16109397, PubMed:16516839). Mediates Wnt ligand egl-20 in the control of the anterior-posterior axon guidance of AVM and PVM neurons (PubMed:16516839).Curated14 Publications

GO - Molecular functioni

  • Wnt-activated receptor activity Source: WormBase

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • embryonic digestive tract morphogenesis Source: UniProtKB
  • embryonic morphogenesis Source: UniProtKB
  • endodermal cell fate specification Source: WormBase
  • engulfment of apoptotic cell Source: WormBase
  • establishment of mitotic spindle orientation Source: UniProtKB
  • gastrulation Source: WormBase
  • interneuron migration Source: UniProtKB
  • left/right axis specification Source: UniProtKB
  • motor neuron migration Source: UniProtKB
  • neuroblast migration Source: UniProtKB
  • neuron migration Source: UniProtKB
  • positive regulation of anterior/posterior axon guidance Source: UniProtKB
  • positive regulation of distal tip cell migration Source: UniProtKB
  • positive regulation of engulfment of apoptotic cell Source: UniProtKB
  • positive regulation of motor neuron migration Source: UniProtKB
  • positive regulation of sensory neuron axon guidance Source: UniProtKB
  • sensory neuron migration Source: UniProtKB
  • Wnt signaling pathway Source: WormBase
  • Wnt signaling pathway, regulating spindle positioning Source: WormBase

Keywordsi

Molecular functionDevelopmental protein, Receptor
Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mom-51 Publication
Gene namesi
Name:mom-51 PublicationImported
ORF Names:T23D8.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiT23D8.1a; CE14048; WBGene00003397; mom-5.
T23D8.1b; CE50472; WBGene00003397; mom-5.

Subcellular locationi

  • Cell membrane 2 Publications; Multi-pass membrane protein Sequence analysis
  • Early endosome 2 Publications

  • Note: Uniformaly localized along the cell membrane throughout the cell cycle, but occasionally enriched at prophase towards the posterior pole of the cell (PubMed:15620652). Sequestered from the cell membrane to endosomes by plr-1 to prevent Wnt signaling (PubMed:24401370).2 Publications

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 230ExtracellularCuratedAdd BLAST214
Transmembranei231 – 251Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini252 – 264CytoplasmicCuratedAdd BLAST13
Transmembranei265 – 285Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini286 – 319ExtracellularCuratedAdd BLAST34
Transmembranei320 – 340Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini341 – 348CytoplasmicCurated8
Transmembranei349 – 369Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini370 – 395ExtracellularCuratedAdd BLAST26
Transmembranei396 – 416Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini417 – 449CytoplasmicCuratedAdd BLAST33
Transmembranei450 – 470Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini471 – 515ExtracellularCuratedAdd BLAST45
Transmembranei516 – 536Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini537 – 570CytoplasmicCuratedAdd BLAST34

GO - Cellular componenti

  • early endosome Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: WormBase

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal with severely defective embryonic morphogenesis (PubMed:9288749, PubMed:9288750, PubMed:15620652). Embryos have defective mitotic spindle orientation in the 8-cell stage ABar blastomere (PubMed:9288749, PubMed:9288750, PubMed:20126385). Furthermore, there is disrupted asymmetric distribution of the transcription factor pop-1 and the disheveled homolog dsh-2, which are required for cell fate decisions in 8-cell stage ABar blastomeres and their descendants (PubMed:12810601, PubMed:15990090). There is also abnormal distal tip cell migration along the anterior-posterior axis of the body and defective clearance of apoptotic cell corpses in embryos (PubMed:20126385, PubMed:26292279). Both double knockout with unc-5 RNAi and knockdown by RNAi in an unc-40 mutant background suppresses the migratory defect of distal tip cells in the mom-5 and unc-40 single mutants (PubMed:26292279). Double knockout with Wnt receptor cfz-2 results in enhanced CAN neuron migration defects and also rescues the ALM migration defects in the single cfz-2 knockout (PubMed:16109397). Double knockout with Wnt receptor mig-1 results in enhanced HSN neuron migration defects and also in anterior-posterior axon guidance defects of PVM and AVM touch neurons (PubMed:16516839). RNAi-mediated knockdown results in embryonic lethality, but surviving animals display morphogenesis defects and PHA phasmid neuron duplication (PubMed:15990090).9 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343K → R: Abolishes sequestration in endosomes by plr-1; when associated with R-430; R-442; R-445; R-541 and R548. 1 Publication1
Mutagenesisi430K → R: Abolishes sequestration in endosomes by plr-1; when associated with R-343; R-442; R-445; R-541 and R548. 1 Publication1
Mutagenesisi442K → R: Abolishes sequestration in endosomes by plr-1; when associated with R-343; R-430; R-445; R-541 and R548. 1 Publication1
Mutagenesisi445K → R: Abolishes sequestration in endosomes by plr-1; when associated with R-343; R-430; R-442; R-541 and R548. 1 Publication1
Mutagenesisi541K → R: Abolishes sequestration in endosomes by plr-1; when associated with R-343; R-430; R-442; R-445 and R548. 1 Publication1
Mutagenesisi548K → R: Abolishes sequestration in endosomes by plr-1; when associated with R-343; R-430; R-442; R-445 and R541. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_500549395817 – 570Protein mom-5CuratedAdd BLAST554

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 98PROSITE-ProRule annotation
Disulfide bondi45 ↔ 91PROSITE-ProRule annotation
Glycosylationi51N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi82 ↔ 119PROSITE-ProRule annotation
Disulfide bondi108 ↔ 145PROSITE-ProRule annotation
Disulfide bondi112 ↔ 136PROSITE-ProRule annotation
Glycosylationi149N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Developmental stagei

Expressed in the 32-cell stage ABar blastomere and onwards into late embryogenesis (PubMed:15620652, PubMed:24401370). In late embryogenesis, enriched at the leading edges of postmitotic epidermal cells during ventral enclosure (PubMed:15620652). Asymmetrically expressed during the larval stages of development in cells of the gonad, vulva, tail, nervous system and hypodermis (PubMed:15620652). Expressed predominantly in QR neuroblast descendants and to a lesser extent in QL neuroblast descendants during the larval stages of development (PubMed:25373777). Expressed highly in anterior P-cells during larval development (PubMed:23295860). Post-embryonically, it is up-regulated at the transition phase after dorsal-ventral migration of distal tip cells and at the beginning of their anterior-posterior migration (PubMed:26292279).5 Publications

Gene expression databases

BgeeiWBGene00003397.

Interactioni

Protein-protein interaction databases

STRINGi6239.T23D8.1.

Structurei

3D structure databases

SMRiA0A0K3AWM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 148FZPROSITE-ProRule annotationAdd BLAST117

Domaini

The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3577. Eukaryota.
ENOG410XRC8. LUCA.
GeneTreeiENSGT00760000118864.
KOiK02432.
OMAiKTLNSWR.
OrthoDBiEOG091G0N5M.

Family and domain databases

InterProiView protein in InterPro
IPR015526. Frizzled/SFRP.
IPR000539. Frizzled/Smoothened_TM.
IPR020067. Frizzled_dom.
IPR017981. GPCR_2-like.
PANTHERiPTHR11309. PTHR11309. 1 hit.
PfamiView protein in Pfam
PF01534. Frizzled. 1 hit.
PF01392. Fz. 1 hit.
PRINTSiPR00489. FRIZZLED.
SMARTiView protein in SMART
SM00063. FRI. 1 hit.
SM01330. Frizzled. 1 hit.
SUPFAMiSSF63501. SSF63501. 1 hit.
PROSITEiView protein in PROSITE
PS50038. FZ. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform bImported (identifier: A0A0K3AWM6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRHILILFL FGCLSADQRL SSTSISSMNG FSTTRKCEHI TIPMCKNLDY
60 70 80 90 100
NQTVFPNLLG HTTQSEAGPA IAQFNPLIKV KCSEDIRLFL CTVYAPVCTV
110 120 130 140 150
LEKPIQPCRE LCLSAKNGCE SLMKKFGFQW PDQLDCNKFP VTDLCVGKNS
160 170 180 190 200
SESSNSKNYR SSNDVTFGVS TIANEVVLSP KKCPHHMHTT SGSHFSLPLL
210 220 230 240 250
SGRLPECSLT CEADNQVPMM FDGRVRRILR IWTAAWSVAC FVCSLFTLVT
260 270 280 290 300
FLVDLSRFAY PVRPILYLAF CYLAISTVYM IGVVGEDGFA CGTYGSTPTT
310 320 330 340 350
LVTQGGENVG CSALAVVHYF FFMSSCAWWL VLCLAWFLAA NLKWGAESIA
360 370 380 390 400
ALSPYFHAMC WGVPAVLSVT VLVTNSVDGD VFTGICSVGN LNPSALVYFF
410 420 430 440 450
FTPIVVSLAL GAVLLVCGIW SMIRIRSYIK LQHADVERNI SKLEKLMLRI
460 470 480 490 500
GAFAIMYSLP TAMNAAIMWY QAVNMPAWLE GWLHHRCVRL QDRELFGFTY
510 520 530 540 550
PVDDCPMDPK VAAPEIIVFL LKYVSQLVVG ITCAIWVVSS KTLSSYHKAY
560 570
LALSSRSPTV PAHVDQVNMR
Length:570
Mass (Da):63,198
Last modified:November 11, 2015 - v1
Checksum:i930A0AF0A83F3B7D
GO
Isoform aImported (identifier: A0A0K3AWM6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-160: NYR → K

Show »
Length:568
Mass (Da):62,893
Checksum:i8CAC485247355F87
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058705158 – 160NYR → K in isoform a. Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013953 mRNA. Translation: AAC47750.1.
BX284601 Genomic DNA. Translation: CAB03398.1.
BX284601 Genomic DNA. Translation: CTQ86376.1.
PIRiT25162.
RefSeqiNP_001300437.1. NM_001313508.1. [A0A0K3AWM6-1]
NP_492635.1. NM_060234.4. [A0A0K3AWM6-2]
UniGeneiCel.19627.

Genome annotation databases

EnsemblMetazoaiT23D8.1a; T23D8.1a; WBGene00003397. [A0A0K3AWM6-2]
T23D8.1b; T23D8.1b; WBGene00003397. [A0A0K3AWM6-1]
GeneIDi172856.
KEGGicel:CELE_T23D8.1.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMOM5_CAEEL
AccessioniPrimary (citable) accession number: A0A0K3AWM6
Secondary accession number(s): G5EEF7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 18, 2017
Last sequence update: November 11, 2015
Last modified: August 30, 2017
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families