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Protein

Chorismate synthase

Gene

aroC

Organism
Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_02860), Phospho-2-dehydro-3-deoxyheptonate aldolase (WI95_29895), Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_14265), Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_21645), Phospho-2-dehydro-3-deoxyheptonate aldolase (Bcep18194_A3826), Phospho-2-dehydro-3-deoxyheptonate aldolase (VL00_13870), Phospho-2-dehydro-3-deoxyheptonate aldolase (Bcep18194_B0086)
  2. 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK), Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC), Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48NADPUniRule annotation1
Binding sitei54NADPUniRule annotation1
Binding sitei278FMN; via amide nitrogenUniRule annotation1
Binding sitei319FMNUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 127FMNUniRule annotation3
Nucleotide bindingi238 – 239FMNUniRule annotation2
Nucleotide bindingi293 – 297FMNUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesisUniRule annotation
LigandFADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
ORF Names:VL00_16715Imported
OrganismiBurkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)Imported
Taxonomic identifieri482957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000036081 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiView protein in InterPro
IPR000453. Chorismate_synth.
IPR035904. Chorismate_synth_AroC_sf.
IPR020541. Chorismate_synthase_CS.
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiView protein in Pfam
PF01264. Chorismate_synt. 1 hit.
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiView protein in PROSITE
PS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0J5VPZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGNTLGTLF TVTTFGESHG PAIGCVIDGC PPGMGLTEAD IQVELDRRKP
60 70 80 90 100
GTSRHVTQRQ EADQVEILSG VFEGVTTGTP IALLIRNTDQ RSKDYGNIVE
110 120 130 140 150
TFRPGHADYT YWQKYGIRDY RGGGRSSARL TAPIVGAGAV AKKWLRERFG
160 170 180 190 200
VEVRGYMSGL GEIDVPFVDW SHVHENPFFS PNAAVVPELE AYMDALRKDG
210 220 230 240 250
DSIGARIDVV ASGVPVGWGE PVFDRLDADI AKAMMSINAV KGVEIGAGFD
260 270 280 290 300
SVAQRGSMHG DELTPGGFVG NHAGGVLGGI STGQDITVSI AIKPTSSIRT
310 320 330 340 350
PRRSITKAGD EATVETFGRH DPCVGIRATP IAESMLALVL IDHALRHRAQ
360
CGDVETSTPK IAGSAT
Length:366
Mass (Da):38,872
Last modified:October 14, 2015 - v1
Checksum:iD1A50C60CE5AD97E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LDWF01000037 Genomic DNA. Translation: KML14790.1.
RefSeqiWP_021160805.1. NZ_LDWP01000056.1.

Genome annotation databases

EnsemblBacteriaiKML14790; KML14790; VL00_16715.
PATRICifig|482957.23.peg.4711.

Similar proteinsi

Entry informationi

Entry nameiA0A0J5VPZ7_BURL3
AccessioniPrimary (citable) accession number: A0A0J5VPZ7
Entry historyiIntegrated into UniProtKB/TrEMBL: October 14, 2015
Last sequence update: October 14, 2015
Last modified: October 25, 2017
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported