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Protein

Glutamyl-tRNA reductase

Gene

hemA

Organism
Mycobacterium haemophilum
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).UniRule annotationSAAS annotation

Miscellaneous

During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.UniRule annotation

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.UniRule annotationSAAS annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (hemA)
  2. Glutamate-1-semialdehyde 2,1-aminomutase (hemL)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei50NucleophileUniRule annotation1
Sitei99Important for activityUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei120SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi189 – 194NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processPorphyrin biosynthesisUniRule annotationSAAS annotation
LigandNADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00251; UER00316.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductaseUniRule annotationSAAS annotation (EC:1.2.1.70UniRule annotationSAAS annotation)
Short name:
GluTRUniRule annotation
Gene namesi
Name:hemAUniRule annotation
ORF Names:ABH38_05495Imported
OrganismiMycobacterium haemophilumImported
Taxonomic identifieri29311 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000036334 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 156GlutR_NInterPro annotationAdd BLAST150
Domaini172 – 313Shikimate_DHInterPro annotationAdd BLAST142
Domaini328 – 426GlutR_dimerInterPro annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 52Substrate bindingUniRule annotation4
Regioni114 – 116Substrate bindingUniRule annotation3

Domaini

Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.UniRule annotation

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.30.460.30. 1 hit.
HAMAPiMF_00087. Glu_tRNA_reductase. 1 hit.
InterProiView protein in InterPro
IPR000343. 4pyrrol_synth_GluRdtase.
IPR015896. 4pyrrol_synth_GluRdtase_dimer.
IPR015895. 4pyrrol_synth_GluRdtase_N.
IPR018214. GluRdtase_CS.
IPR036453. GluRdtase_dimer_dom_sf.
IPR036343. GluRdtase_N_sf.
IPR036291. NAD(P)-bd_dom_sf.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
PfamiView protein in Pfam
PF00745. GlutR_dimer. 1 hit.
PF05201. GlutR_N. 1 hit.
PF01488. Shikimate_DH. 1 hit.
PIRSFiPIRSF000445. 4pyrrol_synth_GluRdtase. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF69075. SSF69075. 1 hit.
SSF69742. SSF69742. 1 hit.
TIGRFAMsiTIGR01035. hemA. 1 hit.
PROSITEiView protein in PROSITE
PS00747. GLUTR. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0I9U712-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILLFGVSH RSAPVSVLEQ LSLDEPDQIK IVDRVLQSPL VTEAMVLSTC
60 70 80 90 100
NRVEVYAVVD AFHAGLSVIG QVLSEYSAMS IGDLTKYAYV RYSEAAVEHL
110 120 130 140 150
FAVTSGLDSA VVGEQQVLGQ VRRAYAAAEA NRAVGRVLHE VAQRALSVGK
160 170 180 190 200
RVHSETAIDA AGASVVSVAL GMAERTLGGL AGRTAVVIGA GAMGALSSSH
210 220 230 240 250
LTQANIGRIK VLNRSLSRAR RLADKIGESG VPAEARTLDQ LAEALANADL
260 270 280 290 300
VVSCTGAVSP VVSLADVHHA LAAMRRDETT QPLVICDLGM PRDVDPAVAK
310 320 330 340 350
LPGVWVVDVD GVQREPSAHA TAADVDAARR IVAAEVATYL AGQRMAEVTP
360 370 380 390 400
TVTALRQRAA DVVEAELLRL DHRLPGLDSG QREEVARTVR RVVDKLLHAP
410 420 430 440 450
TVRIKQLASA PGGDSYAEAL RELFELDQTA VDAVATAGEL PVVASGFDDA
460
IRHGTSAAQG SSKHPTE
Length:467
Mass (Da):49,068
Last modified:October 14, 2015 - v1
Checksum:i39A27D4EF28E9630
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LDPR01000003 Genomic DNA. Translation: KLO38046.1.
RefSeqiWP_047313630.1. NZ_LDPT01000007.1.

Genome annotation databases

EnsemblBacteriaiKLO38046; KLO38046; ABH38_05495.
PATRICifig|29311.18.peg.1859.

Similar proteinsi

Entry informationi

Entry nameiA0A0I9U712_9MYCO
AccessioniPrimary (citable) accession number: A0A0I9U712
Entry historyiIntegrated into UniProtKB/TrEMBL: October 14, 2015
Last sequence update: October 14, 2015
Last modified: March 28, 2018
This is version 16 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported