ddl_1

Functionⁱ

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityⁱ

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactorⁱ

Protein has several cofactor binding sites:

Mg²⁺
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRSR:PIRSR039102-3
, Mn²⁺
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRSR:PIRSR039102-3
Note: Binds 2 magnesium or manganese ions per subunit.
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRSR:PIRSR039102-3
Mn²⁺
SAAS annotation
Automatic assertion according to rulesⁱ
- SAAS:SAAS00910564

Pathwayⁱ: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	257	Magnesium or manganese 1UniRule annotation	1
Metal bindingⁱ	270	Magnesium or manganese 1UniRule annotation	1
Metal bindingⁱ	270	Magnesium or manganese 2UniRule annotation	1
Metal bindingⁱ	272	Magnesium or manganese 2UniRule annotation	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-UniRule
D-alanine-D-alanine ligase activity Source: UniProtKB-UniRule
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall organization Source: UniProtKB-KW
peptidoglycan biosynthetic process Source: UniProtKB-UniRule
regulation of cell shape Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	LigaseUniRule annotationSAAS annotationImported
Biological process	Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
Ligand	ATP-bindingPROSITE-ProRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00219.

UniPathwayⁱ

UPA00219.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: D-alanine--D-alanine ligaseUniRule annotationSAAS annotation (EC:6.3.2.4UniRule annotationSAAS annotation) Alternative name(s): D-Ala-D-Ala ligaseUniRule annotation D-alanylalanine synthetaseUniRule annotation
Gene namesⁱ	Name:ddl_1Imported Synonyms:ddlUniRule annotationImported ORF Names:BZ172_16140Imported, CI633_25585Imported, CI726_03015Imported, ERS428554_00689Imported
Organismⁱ	Shigella sonneiImported
Taxonomic identifierⁱ	624 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Shigella
Proteomesⁱ	UP000194501 Componentⁱ: Chromosome UP000045991 Componentⁱ: Unassembled WGS sequence UP000215054 Componentⁱ: Unassembled WGS sequence UP000215497 Componentⁱ: Unassembled WGS sequence

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Automatic assertion according to rulesⁱ
- HAMAP-Rule:MF_00047
SAAS annotation
Automatic assertion according to rulesⁱ
- SAAS:SAAS00644680

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

CytoplasmUniRule annotationSAAS annotation

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	101 – 303	ATP-graspInterPro annotationAdd BLAST		203

Sequence similaritiesⁱ

Belongs to the D-alanine--D-alanine ligase family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Dⁱ	3.30.1490.20. 1 hit.
HAMAPⁱ	MF_00047. Dala_Dala_lig. 1 hit.
InterProⁱ	View protein in InterPro IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFⁱ	PIRSF039102. Ddl/VanB. 2 hits.
SUPFAMⁱ	SSF52440. SSF52440. 1 hit.
TIGRFAMsⁱ	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

A0A0I2EZP5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTDKIAVLLG GTSAEREVSL NSGAAVLAGL REGGIDAYPV DPKEVDVTQL 
        60         70         80         90        100
KSMGFQKVFI ALHGRGGEDG TLQGMLELMG LPYTGSGVMA SALSMDKLRS 
       110        120        130        140        150
KLLWQGAGLP VAPWVALTRA EFEKGLSDKQ LAEISALGLP VIVKPSREGS 
       160        170        180        190        200
SVGMSKVVAE NALQDALRLA FQHDEEVLIE KWLSGPEFTV AILGEEILPS 
       210        220        230        240        250
IRIQPSGTFY DYEAKYLSDE TQYFCPAGLE ASQEANLQAL VLKAWTTLGC 
       260        270        280        290        300
KGWGRIDVML DSDGQFYLLE ANTSPGMTSH SLVPMAARQA GMSFSQLVVR 

ILELAD

Length:306

Mass (Da):32,840

Last modified:October 14, 2015 - v1

Checksum:ⁱF2D401C323A04471

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP019689 Genomic DNA. Translation: ARS06705.1. CWXZ01000009 Genomic DNA. Translation: CSK29440.1. NPZM01000159 Genomic DNA. Translation: OYE46128.1. NQBH01000015 Genomic DNA. Translation: OYI11964.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000130056.1. NZ_NVQG01000012.1.

Genome annotation databases

EnsemblBacteriaⁱ	CSK29440; CSK29440; ERS428554_00689. CSM33749; CSM33749; ERS432308_00374.
PATRICⁱ	fig\|624.635.peg.108.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A0I2EZP5	P07862 A0A024KMB8 A0A1S6T6B9 J7QWX0 A0A080JHL2 A0A238BEP1 I4T5Q3 F4SF95 A0A025CKI8 A0A023YRZ7 +100	Escherichia coli (strain K12) Escherichia coli D6-113.11 Escherichia coli M8 Escherichia coli Escherichia coli 1-250-04_S3_C2 Shigella boydii Escherichia coli 541-15 Escherichia coli H736 Escherichia coli O145:NM str. 2010C-3526 Escherichia coli O145:H28 str. RM12581 And more	306	UniRef100_P07862	Cluster: D-alanine--D-alanine ligase B	111

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A0I2EZP5	P07862 A0A024KMB8 A0A1S6T6B9 J7QWX0 A0A080JHL2 A0A238BEP1 I4T5Q3 F4SF95 A0A025CKI8 A0A023YRZ7 +720	Escherichia coli (strain K12) Escherichia coli D6-113.11 Escherichia coli M8 Escherichia coli Escherichia coli 1-250-04_S3_C2 Shigella boydii Escherichia coli 541-15 Escherichia coli H736 Escherichia coli O145:NM str. 2010C-3526 Escherichia coli O145:H28 str. RM12581 And more	306	UniRef90_P07862	Cluster: D-alanine--D-alanine ligase B	731

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
A0A0I2EZP5	P07862 A0A024KMB8 A0A1S6T6B9 J7QWX0 A0A080JHL2 A0A238BEP1 I4T5Q3 F4SF95 A0A025CKI8 A0A023YRZ7 +1257	Escherichia coli (strain K12) Escherichia coli D6-113.11 Escherichia coli M8 Escherichia coli Escherichia coli 1-250-04_S3_C2 Shigella boydii Escherichia coli 541-15 Escherichia coli H736 Escherichia coli O145:NM str. 2010C-3526 Escherichia coli O145:H28 str. RM12581 And more	306	UniRef50_P07862	Cluster: D-alanine--D-alanine ligase B	1,268

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP019689 Genomic DNA. Translation: ARS06705.1. CWXZ01000009 Genomic DNA. Translation: CSK29440.1. NPZM01000159 Genomic DNA. Translation: OYE46128.1. NQBH01000015 Genomic DNA. Translation: OYI11964.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000130056.1. NZ_NVQG01000012.1.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CSK29440; CSK29440; ERS428554_00689. CSM33749; CSM33749; ERS432308_00374.
PATRICⁱ	fig\|624.635.peg.108.

Enzyme and pathway databases

UniPathwayⁱ	UPA00219.

UniPathwayⁱ

UPA00219.

Family and domain databases

Gene3Dⁱ	3.30.1490.20. 1 hit.
HAMAPⁱ	MF_00047. Dala_Dala_lig. 1 hit.
InterProⁱ	View protein in InterPro IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFⁱ	PIRSF039102. Ddl/VanB. 2 hits.
SUPFAMⁱ	SSF52440. SSF52440. 1 hit.
TIGRFAMsⁱ	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	A0A0I2EZP5_SHISO
Accessionⁱ	A0A0I2EZP5Primary (citable) accession number: A0A0I2EZP5
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	October 14, 2015
	Last sequence update:	October 14, 2015
	Last modified:	January 31, 2018
	This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteomeImported

UniProtKB - A0A0I2EZP5 (A0A0I2EZP5_SHISO)

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D-alanine--D-alanine ligase

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Pathwayⁱ: peptidoglycan biosynthesis

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Enzyme and pathway databases

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Family & Domainsⁱ

Domains and Repeats

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - A0A0I2EZP5 (A0A0I2EZP5_SHISO)

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D-alanine--D-alanine ligase

ddl_1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: peptidoglycan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ