Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fatty acid oxidation complex subunit alpha

Gene

fadB

Organism
Pseudomonas aeruginosa str. Stone 130
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotationSAAS annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotationSAAS annotation
(3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.UniRule annotationSAAS annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotationSAAS annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotationSAAS annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei120Important for catalytic activityUniRule annotation1
Sitei140Important for catalytic activityUniRule annotation1
Binding sitei297SubstrateUniRule annotation1
Binding sitei325NAD; via amide nitrogenUniRule annotation1
Binding sitei344NADUniRule annotation1
Binding sitei408NADUniRule annotation1
Binding sitei430NADUniRule annotation1
Active sitei451For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation1
Binding sitei454NADUniRule annotation1
Binding sitei501SubstrateUniRule annotation1
Binding sitei660SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi401 – 403NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotation, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation, OxidoreductaseUniRule annotationSAAS annotation
Biological processFatty acid metabolismUniRule annotationSAAS annotation, Lipid degradationUniRule annotationSAAS annotation, Lipid metabolism
LigandNADUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation, EC:5.3.3.8UniRule annotation)
Gene namesi
Name:fadBUniRule annotation
ORF Names:HMPREF1223_08146Imported
OrganismiPseudomonas aeruginosa str. Stone 130Imported
Taxonomic identifieri1125697 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000017043 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0H3R7M5.
SMRiA0A0H3R7M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini318 – 4953HCDH_NInterPro annotationAdd BLAST178
Domaini497 – 5923HCDHInterPro annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST190
Regioni312 – 7153-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST404

Sequence similaritiesi

In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotationSAAS annotation

Phylogenomic databases

OrthoDBiPOG091H00UW.

Family and domain databases

HAMAPiMF_01621. FadB. 1 hit.
InterProiView protein in InterPro
IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH-like_C_sf.
IPR029045. ClpP/crotonase-like_dom_sf.
IPR018376. Enoyl-CoA_hyd/isom_CS.
IPR001753. Enoyl-CoA_hydra/iso.
IPR012799. FadB.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF00725. 3HCDH. 1 hit.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH_1. 1 hit.
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF51735. SSF51735. 1 hit.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02437. FadB. 1 hit.
PROSITEiView protein in PROSITE
PS00067. 3HCDH. 1 hit.
PS00166. ENOYL_COA_HYDRATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0H3R7M5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYQGKAITV KPLEGGIVEL NFDLKGESVN KFNRLTLSEL RAAVDAIKAD
60 70 80 90 100
ASVKGVIVTS GKDVFIVGAD ITEFVDNFQL PDEELMAGNL EANKIFSDFE
110 120 130 140 150
DLDVPTVAAI NGIALGGGLE MCLAADFRVM SATAKVGLPE VKLGIYPGFG
160 170 180 190 200
GTVRLPRLIG CDNAVEWIAS GKENKAEDAL KVGAVDAVVA PEQLQAAALD
210 220 230 240 250
LAKRAVAGEL DHKARRQPKL EKLKLNAIEQ MMAFETAKGF VAGQAGPNYP
260 270 280 290 300
APVEAIKSIQ KAANFGRDKA LEVEAAGFVK LAKTSVAQSL IGLFLNDQEL
310 320 330 340 350
KKKAKKYDEV AKDVKLAAVL GAGIMGGGIA YQSALKGTPI LMKDIREEGI
360 370 380 390 400
QMGLNEAAKL LGKRVEKGRL TPAKMAEALN GIRPTMSYGD FGNVDIVVEA
410 420 430 440 450
VVENPKVKQA VLAEVEGAVK EDAIIASNTS TISISLLAQA LKRPENFCGM
460 470 480 490 500
HFFNPVHMMP LVEVIRGEKT GETAIATTVA YAKKMGKSPI VVNDCPGFLV
510 520 530 540 550
NRVLFPYFGG FAKLLSFGVD FVRIDKVMEK FGWPMGPAYL SDVVGIDTGH
560 570 580 590 600
HGRDVMAEGF PDRMAVEGKT AVDVMYEANR LGQKNGKGFY AYETDKRGKP
610 620 630 640 650
KKVTDPQAYE VLKPIVVEQR EVTDEDIVNF MMIPLCLETV RCLEDGIVET
660 670 680 690 700
AAEADMGLIY GIGFPPFRGG ALRYIDSIGV AEFVALADKY AELGALYHPT
710
AKLREMAKNG QKFFG
Length:715
Mass (Da):76,954
Last modified:September 16, 2015 - v1
Checksum:i7ED8573BE8F31101
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AQFN01000012 Genomic DNA. Translation: EMZ57747.1.
RefSeqiWP_003091204.1. NZ_KI517348.1.

Genome annotation databases

EnsemblBacteriaiEMZ57747; EMZ57747; HMPREF1223_08146.
PATRICifig|1125697.3.peg.1145.

Similar proteinsi

Entry informationi

Entry nameiA0A0H3R7M5_PSEAI
AccessioniPrimary (citable) accession number: A0A0H3R7M5
Entry historyiIntegrated into UniProtKB/TrEMBL: September 16, 2015
Last sequence update: September 16, 2015
Last modified: February 28, 2018
This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported