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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Peptoclostridium difficile (strain CD196) (Clostridium difficile)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotation, Transferase
Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotationImported
Ordered Locus Names:CD196_0108Imported
OrganismiPeptoclostridium difficile (strain CD196) (Clostridium difficile)Imported
Taxonomic identifieri645462 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000002068 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymeraseUniRule annotationImported

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliA0A0H3MYZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 227RPOLDInterPro annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 228Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST228
Regioni247 – 315Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST69

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

KOiK03040.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
3.30.1360.10. 2 hits.
HAMAPiMF_00059. RNApol_bact_RpoA. 1 hit.
InterProiView protein in InterPro
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR036603. RBP11-like.
IPR011260. RNAP_asu_C.
IPR036643. RNApol_insert_sf.
PfamiView protein in Pfam
PF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179. RNAP_asu_C. 1 hit.
SMARTiView protein in SMART
SM00662. RPOLD. 1 hit.
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0H3MYZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIEKPKVD IVELSEDYRY GKFVIEPLER GYGITIGNAL RRILLSSLPG
60 70 80 90 100
VAVNAIKIDG VLHEFSTIPG VKEDVTEIIL TLKELSATID GEGSRTLKIE
110 120 130 140 150
AQGPCSITGA DIICPPDVEI LSKDLAIATL DDNAKLNMEI FVDKGRGYVS
160 170 180 190 200
AEENKTENVP IGVLPVDSIY TPVEKVSYHV ENTRVGQKTD YDKLVLEVWT
210 220 230 240 250
NGSINPQEGI SLAAKVLVEH LNLFIDLTEH VSSVEIMVEK EEDQKEKVLE
260 270 280 290 300
MTIEELDLSV RSYNCLKRAG INTVEELANK SEDDMMKVRN LGKKSLEEVI
310
QKLEELGLGL KPSEE
Length:315
Mass (Da):34,920
Last modified:September 16, 2015 - v1
Checksum:iE75526C3C02D9B16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN538970 Genomic DNA. Translation: CBA60238.1.
RefSeqiWP_003427733.1. NC_013315.1.

Genome annotation databases

EnsemblBacteriaiCBA60238; CBA60238; CD196_0108.
GeneIDi31351592.
KEGGicdc:CD196_0108.

Similar proteinsi

Entry informationi

Entry nameiA0A0H3MYZ4_PEPDC
AccessioniPrimary (citable) accession number: A0A0H3MYZ4
Entry historyiIntegrated into UniProtKB/TrEMBL: September 16, 2015
Last sequence update: September 16, 2015
Last modified: February 28, 2018
This is version 17 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported