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Protein

Trehalose 2-sulfotransferase

Gene

stf0

Organism
Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the sulfuryl group transfer from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to trehalose, leading to trehalose-2-sulfate (T2S). The sulfation of trehalose is the first step in the biosynthesis of sulfolipid-1 (SL-1), a major cell wall glycolipid and the most abundant sulfated metabolite found in Mycobacterium tuberculosis, that is a potential virulence factor thought to mediate host-pathogen interactions.By similarity1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose.By similarity

Pathwayi: Glycolipid metabolism

This protein is involved in Glycolipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in Glycolipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14alpha,alpha-trehaloseBy similarity1
Active sitei36Proton acceptorBy similarity1
Binding sitei48alpha,alpha-trehalose; via carbonyl oxygenBy similarity1
Binding sitei53alpha,alpha-trehaloseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose 2-sulfotransferaseBy similarity (EC:2.8.2.37By similarity)
Gene namesi
Name:stf01 Publication
Ordered Locus Names:ERDMAN_0329Imported
OrganismiMycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)
Taxonomic identifieri652616 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000007568 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Loss of T2S and SL-1 formation, but phthiocerol dimycocerosate (PDIM) biosynthesis remains intact. Cells lacking this gene exhibit augmented survival in human but not murine macrophages, suggesting that SL-1 negatively regulates the intracellular growth of M.tuberculosis in a species-specific manner. Moreover, the mutant strains exhibit increased resistance in vitro to a human cationic antimicrobial peptide, LL-37, while they do not show global defects in overall cell envelope integrity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004347841 – 267Trehalose 2-sulfotransferaseAdd BLAST267

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA0A0H3L952.
SMRiA0A0H3L952.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 39alpha,alpha-trehalose bindingBy similarity7

Sequence similaritiesi

Belongs to the Stf0 sulfotransferase family.Curated

Phylogenomic databases

KOiK21014.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR015124. Stf0.
IPR024628. Sulfotransferase_Stf0_dom.
PfamiView protein in Pfam
PF09037. Sulphotransf. 1 hit.
PIRSFiPIRSF021497. Sulphotransferase_Stf0. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0H3L952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRAVRPYLV LATQRSGSTL LVESLRATGC AGEPQEFFQY LPSTGMAPQP
60 70 80 90 100
REWFAGVDDD TILQLLDPLD PGTPDTATPV AWREHVRTSG RTPNGVWGGK
110 120 130 140 150
LMWNQTALLQ QRAAQLPDRS GDGLRAAIRD VIGNEPVFVH VHRPDVVSQA
160 170 180 190 200
VSFWRAVQTQ VWRGHPDPKR DSQAVYHAGA IAHIIRNLRD QENGWRAWFA
210 220 230 240 250
EEGIDPIDIA YPVLWRNLTA IVASVLDAIG QDPKLAPAPM LERQANQRSD
260
EWVDRYRAEA PRLGLPT
Length:267
Mass (Da):29,775
Last modified:November 11, 2015 - v2
Checksum:iE60286DC61C5267C
GO

Sequence cautioni

The sequence BAL64146 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012340 Genomic DNA. Translation: BAL64146.1. Different initiation.
RefSeqiWP_003401540.1. NZ_KK339487.1.

Genome annotation databases

EnsemblBacteriaiBAL64146; BAL64146; ERDMAN_0329.
KEGGimtn:ERDMAN_0329.
PATRICifig|652616.3.peg.334.

Similar proteinsi

Entry informationi

Entry nameiSTF0_MYCTE
AccessioniPrimary (citable) accession number: A0A0H3L952
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 11, 2015
Last sequence update: November 11, 2015
Last modified: June 7, 2017
This is version 11 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families