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Protein

Octanoyltransferase

Gene

lipB

Organism
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.UniRule annotationSAAS annotation

Miscellaneous

In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.UniRule annotation

Catalytic activityi

Octanoyl-[acyl-carrier-protein] + protein = protein N6-(octanoyl)lysine + [acyl-carrier-protein].UniRule annotationSAAS annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 1 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei193Lowers pKa of active site CysUniRule annotation1
Active sitei227Acyl-thioester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotationSAAS annotation, Transferase

Enzyme and pathway databases

UniPathwayiUPA00538; UER00592.

Names & Taxonomyi

Protein namesi
Recommended name:
OctanoyltransferaseUniRule annotationSAAS annotation (EC:2.3.1.181UniRule annotation)
Alternative name(s):
Lipoate-protein ligase BUniRule annotation
Lipoyl/octanoyl transferaseUniRule annotation
Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferaseUniRule annotation
Gene namesi
Name:lipBUniRule annotationImported
Ordered Locus Names:BOV_A0555Imported
OrganismiBrucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)Imported
Taxonomic identifieri444178 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000006383 Componenti: Chromosome II

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0H3AV66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 265BPL/LPL catalyticInterPro annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 123Substrate bindingUniRule annotation8
Regioni196 – 198Substrate bindingUniRule annotation3
Regioni209 – 211Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the LipB family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK03801.
OMAiMHGFAFN.

Family and domain databases

CDDicd16444. LipB. 1 hit.
HAMAPiMF_00013. LipB. 1 hit.
InterProiView protein in InterPro
IPR004143. BPL_LPL_catalytic.
IPR000544. Octanoyltransferase.
IPR020605. Octanoyltransferase_CS.
PfamiView protein in Pfam
PF03099. BPL_LplA_LipB. 1 hit.
PIRSFiPIRSF016262. LPLase. 1 hit.
TIGRFAMsiTIGR00214. lipB. 1 hit.
PROSITEiView protein in PROSITE
PS51733. BPL_LPL_CATALYTIC. 1 hit.
PS01313. LIPB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0H3AV66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTGKLRQKP PYAAIMTNSP VTPSTETQQP KRDALYARFL PQEADAPPVE
60 70 80 90 100
WLIAEGLTDY EEALAFMEAR VQAIREGTAS ELVWLVEHPP LYTAGTSANA
110 120 130 140 150
EDLLTPDRFP VFNTGRGGEY TYHGPGQRVA YVMLDLKRRR EDVRAFVASL
160 170 180 190 200
EQWIIETLAA FNIKGERRED RVGVWVVRSE KPRLADGSMC EDKIAAIGIR
210 220 230 240 250
LRRWVSFHGI AINVEPDLSH YGGIVPCGIS EHGVTSLVDL GLPVTMGDVD
260
VALGKAFESV FGPRQTK
Length:267
Mass (Da):29,591
Last modified:September 16, 2015 - v1
Checksum:i9DFB163473961995
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000709 Genomic DNA. Translation: ABQ62827.1.

Genome annotation databases

EnsemblBacteriaiABQ62827; ABQ62827; BOV_A0555.
KEGGibov:BOV_A0555.

Similar proteinsi

Entry informationi

Entry nameiA0A0H3AV66_BRUO2
AccessioniPrimary (citable) accession number: A0A0H3AV66
Entry historyiIntegrated into UniProtKB/TrEMBL: September 16, 2015
Last sequence update: September 16, 2015
Last modified: October 25, 2017
This is version 16 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported