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Protein

Pyridoxine/pyridoxamine 5'-phosphate oxidase

Gene

pdxH

Organism
Pseudomonas sp. BRH_c35
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).UniRule annotationSAAS annotation

Catalytic activityi

Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.UniRule annotationSAAS annotation
Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.UniRule annotationSAAS annotation

Cofactori

FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Pathwayi: pyridoxal 5'-phosphate salvage

This protein is involved in step 1 of the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxine 5'-phosphate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH)
This subpathway is part of the pathway pyridoxal 5'-phosphate salvage, which is itself part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxal 5'-phosphate from pyridoxine 5'-phosphate, the pathway pyridoxal 5'-phosphate salvage and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69SubstrateUniRule annotation1
Binding sitei86FMNUniRule annotation1
Binding sitei108FMNUniRule annotation1
Binding sitei126SubstrateUniRule annotation1
Binding sitei130SubstrateUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei188FMNUniRule annotation1
Binding sitei198FMNUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 69FMNUniRule annotation6
Nucleotide bindingi79 – 80FMNUniRule annotation2
Nucleotide bindingi143 – 144FMNUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationSAAS annotation
Biological processPyridoxine biosynthesisUniRule annotationSAAS annotation
LigandFlavoprotein, FMNUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA01068; UER00304.
UPA01068; UER00305.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine/pyridoxamine 5'-phosphate oxidaseUniRule annotation (EC:1.4.3.5UniRule annotation)
Alternative name(s):
PNP/PMP oxidaseUniRule annotation
Short name:
PNPOxUniRule annotation
Pyridoxal 5'-phosphate synthaseUniRule annotation
Gene namesi
Name:pdxHUniRule annotation
ORF Names:VR76_07825Imported
OrganismiPseudomonas sp. BRH_c35Imported
Taxonomic identifieri1629711 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000033602 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 122Putative_PNPOxInterPro annotationAdd BLAST83
Domaini175 – 215PNP_phzG_CInterPro annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 12Substrate bindingUniRule annotation4
Regioni194 – 196Substrate bindingUniRule annotation3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili143 – 163Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the pyridoxamine 5'-phosphate oxidase family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

Gene3Di2.30.110.10. 1 hit.
HAMAPiMF_01629. PdxH. 1 hit.
InterProiView protein in InterPro
IPR000659. Pyridox_Oxase.
IPR019740. Pyridox_Oxase_CS.
IPR011576. Pyridox_Oxase_put.
IPR019576. Pyridoxamine_oxidase_dimer_C.
IPR012349. Split_barrel_FMN-bd.
PANTHERiPTHR10851:SF0. PTHR10851:SF0. 1 hit.
PfamiView protein in Pfam
PF10590. PNP_phzG_C. 1 hit.
PF01243. Putative_PNPOx. 1 hit.
PIRSFiPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
SUPFAMiSSF50475. SSF50475. 1 hit.
TIGRFAMsiTIGR00558. pdxH. 1 hit.
PROSITEiView protein in PROSITE
PS01064. PYRIDOX_OXIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0F2QTA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQTLADMRR DYTRDGLSEA NAPAEPFGLF RQWFTEATKT EQAPVEPNAM
60 70 80 90 100
SLATVDSDGH PHCRILLLKA LDDRGFTFFT NYDSAKGEQL NASPYAAMTF
110 120 130 140 150
FWPTLERQVR IEGRVEKVTQ EESDAYFQVR PLGSRLGAWA SPQSQVIRDR
160 170 180 190 200
AELERLLAET EQRFLDTAPH CPPHWGGYRL LPERMEFWQG RPSRLHDRLD
210
YRLIANSWQR ERLAP
Length:215
Mass (Da):24,913
Last modified:June 24, 2015 - v1
Checksum:i3E3EA99A96626513
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LADX01000140 Genomic DNA. Translation: KJS28272.1.

Genome annotation databases

PATRICifig|1629711.3.peg.2384.

Similar proteinsi

Entry informationi

Entry nameiA0A0F2QTA0_9PSED
AccessioniPrimary (citable) accession number: A0A0F2QTA0
Entry historyiIntegrated into UniProtKB/TrEMBL: June 24, 2015
Last sequence update: June 24, 2015
Last modified: December 20, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported