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Protein

Biotin synthase

Gene

bioB

Organism
Yersinia pestis bv. Antiqua (strain Antiqua)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD), ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB), Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi57Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi60Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi97Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi128Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi188Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi260Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotationImported
ORF Names:CH58_3931Imported
OrganismiYersinia pestis bv. Antiqua (strain Antiqua)Imported
Taxonomic identifieri360102 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000031888 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0E1NRG9.
SMRiA0A0E1NRG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 255Elp3InterPro annotationAdd BLAST213
Domaini220 – 312BATSInterPro annotationAdd BLAST93

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

OMAiADRFCMG.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR034416. BATS_domain_containing.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDF00272. biotin_synthase. 1 hit.
SFLDG01060. BATS_domain_containing. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0E1NRG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYHHWTVG QALALFDKPL LELLFEAQQV HRQHFDPRQV QVSTLLSIKT
60 70 80 90 100
GACPEDCKYC PQSSRYKTGL ESERLMQVEQ VLESAKKAKA AGSTRFCMGA
110 120 130 140 150
AWKNPHERDM PYLAKMVEGV KALGMETCMT LGSLSKQQAH RLADAGLDYY
160 170 180 190 200
NHNLDTSPEF YGSIITTRSY QERLDTLNEV RDAGIKVCSG GIVGLGETVR
210 220 230 240 250
DRAGLLVQLA NLPKPPESVP INMLVKVKGT PLENNAEVDA FEFIRTIAVA
260 270 280 290 300
RIMMPSSYVR LSAGREQMNE QTQAMCFMAG ANSIFYGCKL LTTPNPDEDK
310 320 330 340
DLQLFRKLGL NPQQTATSHG DREQQQALTE QLLHGDTAQF YNAAV
Length:345
Mass (Da):38,378
Last modified:May 27, 2015 - v1
Checksum:i5E947F85B26780A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP009906 Genomic DNA. Translation: AJJ79466.1.
RefSeqiWP_002210762.1. NZ_CP009906.1.

Genome annotation databases

EnsemblBacteriaiAJJ79466; AJJ79466; CH58_3931.
PATRICifig|360102.15.peg.4130.

Similar proteinsi

Entry informationi

Entry nameiA0A0E1NRG9_YERPA
AccessioniPrimary (citable) accession number: A0A0E1NRG9
Entry historyiIntegrated into UniProtKB/TrEMBL: May 27, 2015
Last sequence update: May 27, 2015
Last modified: November 22, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported