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Protein

Biotin synthase

Gene

bioB

Organism
Campylobacter fetus subsp. fetus 04/554
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi18Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi22Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi62Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi155Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotationImported
ORF Names:CFF04554_0177Imported
OrganismiCampylobacter fetus subsp. fetus 04/554Imported
Taxonomic identifieri1240980 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000029484 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 213Elp3InterPro annotationAdd BLAST206
Domaini185 – 275BATSInterPro annotationAdd BLAST91

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01012

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0E1GG68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKMVMLCAI CNVSSGNCAE DCAYCTQSAH IKADIPKFKQ KNLEQILNEA
60 70 80 90 100
KIASKNHALG FCLVTSGLGL DDKKLEFICE AATMLRKETP NLMLIACNGS
110 120 130 140 150
ASYESLKELK KVGIFSYNHN LETSREFFPQ ICTTHSWDDR FNTNLNAKKA
160 170 180 190 200
GLELCCGGIY GLGESQDDRL SFRASLKELN PFSSPINFFI PNPALKIKQP
210 220 230 240 250
LLSTDEALEI IIDTAKTLPQ CRIMVAGGRE IVLGDRQYEI LENGASAIVI
260 270 280
GDYLTTSGEV ASKDIEELRN RGFEFASQCH
Length:280
Mass (Da):30,908
Last modified:May 27, 2015 - v1
Checksum:iF3EF582A41300566
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP008808 Genomic DNA Translation: AIR78130.1
RefSeqiWP_038452683.1, NZ_CP008808.1

Genome annotation databases

EnsemblBacteriaiAIR78130; AIR78130; CFF04554_0177
KEGGicft:CFF04554_0177

Similar proteinsi

Entry informationi

Entry nameiA0A0E1GG68_CAMFE
AccessioniPrimary (citable) accession number: A0A0E1GG68
Entry historyiIntegrated into UniProtKB/TrEMBL: May 27, 2015
Last sequence update: May 27, 2015
Last modified: November 22, 2017
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported