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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus aureus subsp. aureus 71193
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei557Aminoacyl-adenylateUniRule annotation1
Binding sitei601ATPUniRule annotation1
Metal bindingi889ZincUniRule annotation1
Metal bindingi892ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi912ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
ORF Names:ST398NM01_1191Imported
OrganismiStaphylococcus aureus subsp. aureus 71193Imported
Taxonomic identifieri1155084 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000005003 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 637tRNA-synt_1InterPro annotationAdd BLAST609
Domaini681 – 834Anticodon_1InterPro annotationAdd BLAST154
Domaini886 – 915zf-FPG_IleRSInterPro annotationAdd BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 70"HIGH" regionUniRule annotationAdd BLAST11
Motifi598 – 602"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01870
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0E0VNE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMDYKETL LMPKTDFPMR GGLPNKEPQI QEKWDAEDQY HKALEKNKGN
60 70 80 90 100
ETFILHDGPP YANGNLHMGH ALNKILKDFI VRYKTMQGFY APYVPGWDTH
110 120 130 140 150
GLPIEQALTK KGVDRKKMST AEFREKCKEF ALEQIELQKK DFRRLGVRGD
160 170 180 190 200
FNDPYITLKP EYEAAQIRIF GEMADKGLIY KGKKPVYWSP SSESSLAEAE
210 220 230 240 250
IEYHDKRSAS IYVAFDVKDD KGVVDADAKF IIWTTTPWTI PSNVAITVHP
260 270 280 290 300
ELKYGQYNVN GEKYIIAEAL SDAVAEALDW DKASIKLEKE YTGKELEYVV
310 320 330 340 350
AQHPFLDRES LVINGYHVTT DAGTGCVHTA PGHGEDDYIV GQKYELPVIS
360 370 380 390 400
PIDDKGVFTE EGGQFEGMFY DKANKAVTDL LTEKGALLKL DFITHSYPHD
410 420 430 440 450
WRTKKPVIFR ATPQWFASIS KVRHDILDAI ENTNFKVNWG KTRIYNMVRD
460 470 480 490 500
RGEWVISRQR VWGVPLPVFY AENGEIIMTK ETVNHVADLF AEHGSNIWFE
510 520 530 540 550
REAKDLLPEG FTHPGSPNGT FTKETDIMDV WFDSGSSHRG VLETRPELSF
560 570 580 590 600
PADMYLEGSD QYRGWFNSSI TTSVATRGVS PYKFLLSHGF VMDGEGKKMS
610 620 630 640 650
KSLGNVIVPD QVVKQKGADI ARLWVSSTDY LADVRISDEI LKQTSDVYRK
660 670 680 690 700
IRNTLRFMLG NINDFNPDTD SIPESELLEV DRYLLNRLRE FTASTINNYE
710 720 730 740 750
NFDYLNIYQE VQNFINVELS NFYLDYGKDI LYIEQRDSHI RRSMQTVLYQ
760 770 780 790 800
ILVDMTKLLA PILVHTAEEV WSHTPHVKEE SVHLADMPKV VEVDQALLDK
810 820 830 840 850
WRTFMNLRDD VNRALETARN EKVIGKSLEA KVTIASNDKF NASEFLTSFD
860 870 880 890 900
ALHQLFIVSQ VKVVDKLDDQ ATAYEHGDIV IEHADGEKCE RCWNYSEDLG
910 920
AVDELTHLCP RCQQVVKSLV
Length:920
Mass (Da):105,330
Last modified:May 27, 2015 - v1
Checksum:i72566D014C38CD1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003045 Genomic DNA Translation: AFH69412.1

Genome annotation databases

EnsemblBacteriaiAFH69412; AFH69412; ST398NM01_1191
KEGGisud:ST398NM01_1191
PATRICifig|1155084.3.peg.1004

Similar proteinsi

Entry informationi

Entry nameiA0A0E0VNE2_STAA5
AccessioniPrimary (citable) accession number: A0A0E0VNE2
Entry historyiIntegrated into UniProtKB/TrEMBL: May 27, 2015
Last sequence update: May 27, 2015
Last modified: March 28, 2018
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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