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Protein

Phosphatidylserine decarboxylase proenzyme, mitochondrial

Gene

PISD

Organism
Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.UniRule annotation

Catalytic activityi

Phosphatidyl-L-serine = phosphatidylethanolamine + CO2.UniRule annotation

Cofactori

pyruvateUniRule annotationNote: Binds 1 pyruvoyl group covalently per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei191Charge relay system; for autoendoproteolytic cleavage activityUniRule annotation1
Active sitei267Charge relay system; for autoendoproteolytic cleavage activityUniRule annotation1
Active sitei378Charge relay system; for autoendoproteolytic cleavage activityUniRule annotation1
Active sitei378Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotation, Lyase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesisUniRule annotation, Phospholipid metabolism
LigandPyruvateUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylserine decarboxylase proenzyme, mitochondrialUniRule annotation (EC:4.1.1.65UniRule annotation)
Cleaved into the following 2 chains:
Phosphatidylserine decarboxylase beta chainUniRule annotation
Phosphatidylserine decarboxylase alpha chainUniRule annotation
Gene namesi
Name:PISDUniRule annotationImported
OrganismiChlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus)Imported
Taxonomic identifieri60711 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeChlorocebus
Proteomesi
  • UP000029965 Componenti: Chromosome 19

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63Mitochondrial matrixUniRule annotationAdd BLAST63
Topological domaini83 – 409Mitochondrial intermembraneUniRule annotationAdd BLAST327

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membraneUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei378Pyruvic acid (Ser); by autocatalysisUniRule annotation1

Post-translational modificationi

Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei377 – 378Cleavage (non-hydrolytic); by autocatalysisUniRule annotation2

Keywords - PTMi

ZymogenUniRule annotation

Interactioni

Subunit structurei

Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type I sub-subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000013484.
OMAiGLFCLNE.

Family and domain databases

HAMAPiMF_03208. PS_decarb_PSD_B_type1_euk. 1 hit.
InterProiView protein in InterPro
IPR003817. PS_Dcarbxylase.
IPR033177. PSD.
IPR033661. PSD_type1_euk.
PANTHERiPTHR10067. PTHR10067. 1 hit.
PfamiView protein in Pfam
PF02666. PS_Dcarbxylase. 1 hit.
TIGRFAMsiTIGR00163. PS_decarb. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0A0D9R8F5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSVVHRFL RLLHGVAPWR SSLHHREITA LSQSLQPLWK LPFRAFRTDA
60 70 80 90 100
RKVHTAPART TFLLRPLPIL LATGGGYAGY RQYEKYRERE LEKLGLEIPP
110 120 130 140 150
KLAGHWEVAL YKSVPTRLLS RAWGRLNQVE LPHWLRRPVY SLYIWTFGVN
160 170 180 190 200
MKEAAVEDLH HYRNLSEFFR RKLKPQARPV CGLHSVISPS DGKILNFGQV
210 220 230 240 250
KNCEVEQVKG VTYSLESFLG PRTYTEDLPF PPAASCDSFK NQLVTREGNE
260 270 280 290 300
LYHCVIYLAP GDYHCFHSPT DWTVSHRRHF PGSLMSVNPG MARWIKELFC
310 320 330 340 350
HNERVVLTGD WKHGFFSLTA VGATNVGSIR IYFDRDLHTN SPRHSKGSYN
360 370 380 390 400
DFSFVTHTNR EGVPMRKGEH LGEFNLGSTI VLIFEAPKDF NFQLKTGQKI

RFGEALGSL
Length:409
Mass (Da):46,910
Last modified:May 27, 2015 - v1
Checksum:i85F3212B2D7193DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AQIB01136778 Genomic DNA. No translation available.
RefSeqiXP_007973662.1. XM_007975471.1.

Genome annotation databases

EnsembliENSCSAT00000006694; ENSCSAP00000004894; ENSCSAG00000008655.
GeneIDi103223189.

Similar proteinsi

Entry informationi

Entry nameiA0A0D9R8F5_CHLSB
AccessioniPrimary (citable) accession number: A0A0D9R8F5
Entry historyiIntegrated into UniProtKB/TrEMBL: May 27, 2015
Last sequence update: May 27, 2015
Last modified: September 27, 2017
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported