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Protein

Thiazole synthase

Gene

thiG

Organism
Bradyrhizobium elkanii
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotationSAAS annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Schiff-base intermediate with DXPUniRule annotation1
Binding sitei157DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processThiamine biosynthesisUniRule annotationSAAS annotation
LigandSchiff baseUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotationSAAS annotation (EC:2.8.1.10UniRule annotationSAAS annotation)
Gene namesi
Name:thiGUniRule annotation
ORF Names:QU41_30905Imported
OrganismiBradyrhizobium elkaniiImported
Taxonomic identifieri29448 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000032215 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 249ThiGInterPro annotationAdd BLAST246

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 185DXP bindingUniRule annotation2
Regioni206 – 207DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0D1NH87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTFYGKSFP SRLLIGTALY PSPAIMQGAI RASGASIVTV SLRREAAGGK
60 70 80 90 100
TGDAFWSLIR ELGVTVLPNT AGCRSVKEAV TTAKLARELF GTPWIKLEVI
110 120 130 140 150
ADNDTLQPDV VGLVEAATIL IKDGFEVFPY CTEDFSVASR LVEAGCKVVM
160 170 180 190 200
PWAAPIGSAK GITNRDSLRL LRDRLPDITL VVDAGLGAPS HAAEALELGY
210 220 230 240 250
DAVLLNTAVA KAADPIAMAN AFRLGVEAGR IAYEAGLMEA RDFASPSTPV
260
VGTPFWHAVS
Length:260
Mass (Da):27,419
Last modified:April 29, 2015 - v1
Checksum:i108B144B14500A2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JXOF01000186 Genomic DNA Translation: KIU44065.1
RefSeqiWP_024579982.1, NZ_JXOF01000186.1

Genome annotation databases

EnsemblBacteriaiKIU44065; KIU44065; QU41_30905
PATRICifig|29448.7.peg.6460

Similar proteinsi

Entry informationi

Entry nameiA0A0D1NH87_BRAEL
AccessioniPrimary (citable) accession number: A0A0D1NH87
Entry historyiIntegrated into UniProtKB/TrEMBL: April 29, 2015
Last sequence update: April 29, 2015
Last modified: December 20, 2017
This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health