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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Ehrlichia minasensis
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi151Zinc 1UniRule annotation1
Metal bindingi154Zinc 1UniRule annotation1
Metal bindingi168Zinc 2UniRule annotation1
Metal bindingi171Zinc 2UniRule annotation1
Metal bindingi190Zinc 2UniRule annotation1
Metal bindingi193Zinc 2UniRule annotation1
Metal bindingi204Zinc 1UniRule annotation1
Metal bindingi207Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri138 – 216CR-typePROSITE-ProRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotation, Stress responseUniRule annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
ORF Names:ehr_00564Imported
OrganismiEhrlichia minasensisImported
Taxonomic identifieri1242993 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
Proteomesi
  • UP000038403 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JInterPro annotationAdd BLAST66
Domaini138 – 216CR-typeInterPro annotationAdd BLAST79
Repeati151 – 158CXXCXGXG motifUniRule annotation8
Repeati168 – 175CXXCXGXG motifUniRule annotation8
Repeati190 – 197CXXCXGXG motifUniRule annotation8
Repeati204 – 211CXXCXGXG motifUniRule annotation8

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili40 – 60Sequence analysisAdd BLAST21

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri138 – 216CR-typePROSITE-ProRule annotationAdd BLAST79

Keywords - Domaini

Coiled coilSequence analysis, RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
IPR036869. J_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0C7D8M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSDYYELL GVSKNATPEE IKKAYRKMAL KYHPDTNPGN KEAEEKFKEL
60 70 80 90 100
SEAYDVLIDQ DKRAAYDKYG HSAFDGGAGR GGFDFNSGFS GDFSDIFNDL
110 120 130 140 150
FGGGFSKGGR SSSRRSEGAV GSDLRFDIEI TLEDSFNGKK VPISYVTYVK
160 170 180 190 200
CSSCSGSGSE GSAKSVQCNT CHGAGSVRTQ QGFFTIERTC HVCNGEGEII
210 220 230 240 250
QNKCKKCSGS GRVRDEVNLL VTIPKGIESG NKIRLNGKGE AGYRGARSGD
260 270 280 290 300
LYVYSNIQKH KFFTRSGSDL YCTVPIKMTL AALGGHIEMP SIDGTWTKVK
310 320 330 340 350
VPEGSQSGDK LRLKEKGMPV INSSKRGDMY IQITVETPVK LTKKQKELLQ
360 370 380
KFDDEPNVDC NPQSTGFFQK VKSFWKDIRS N
Length:381
Mass (Da):41,898
Last modified:April 29, 2015 - v1
Checksum:i6B6759B4BA3617F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CDGH01000060 Genomic DNA. Translation: CEI85178.1.
RefSeqiWP_045171211.1. NZ_CDGH01000060.1.

Similar proteinsi

Entry informationi

Entry nameiA0A0C7D8M2_9RICK
AccessioniPrimary (citable) accession number: A0A0C7D8M2
Entry historyiIntegrated into UniProtKB/TrEMBL: April 29, 2015
Last sequence update: April 29, 2015
Last modified: March 28, 2018
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported