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Protein

Fatty acid desaturase 2

Gene

FADS2

Organism
Tachysurus fulvidraco (Yellow catfish) (Pimelodus fulvidraco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions.By similarity

Catalytic activityi

Linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O.By similarity
Alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O.By similarity

Pathwayi: polyunsaturated fatty acid biosynthesis

This protein is involved in the pathway polyunsaturated fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway polyunsaturated fatty acid biosynthesis and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

Enzyme and pathway databases

UniPathwayiUPA00658.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid desaturase 2Curated (EC:1.14.19.3By similarity)
Alternative name(s):
Acyl-CoA 6-desaturaseCurated
Delta(6) fatty acid desaturase1 Publication
Short name:
Fads2 (delta6)1 Publication
Delta-6 fatty acid desaturaseCurated
Gene namesi
Name:FADS2Curated
Synonyms:Delta6FADImported
OrganismiTachysurus fulvidraco (Yellow catfish) (Pimelodus fulvidraco)Imported
Taxonomic identifieri1234273 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiSiluriformesBagridaeTachysurus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 131CytoplasmicSequence analysisAdd BLAST131
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 158LumenalSequence analysis6
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 265CytoplasmicSequence analysisAdd BLAST86
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Topological domaini287 – 306LumenalSequence analysisAdd BLAST20
Transmembranei307 – 327HelicalSequence analysisAdd BLAST21
Topological domaini328 – 445CytoplasmicSequence analysisAdd BLAST118

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004413971 – 445Fatty acid desaturase 2Add BLAST445

Expressioni

Tissue specificityi

Expression is highest in intestine, followed by brain and heart, and lowest in gill. Also expressed in liver, spleen and muscle.1 Publication

Structurei

3D structure databases

SMRiA0A0C5PRW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 96Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi181 – 185Histidine box-11 Publication5
Motifi218 – 222Histidine box-21 Publication5
Motifi383 – 387Histidine box-31 Publication5

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.Curated

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiView protein in InterPro
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR036400. Cyt_B5-like_heme/steroid_sf.
IPR005804. FA_desaturase_dom.
IPR012171. Fatty_acid_desaturase.
PfamiView protein in Pfam
PF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
PIRSFiPIRSF015921. FA_sphinglp_des. 1 hit.
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiView protein in SMART
SM01117. Cyt-b5. 1 hit.
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiView protein in PROSITE
PS50255. CYTOCHROME_B5_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0C5PRW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGGGQLTEP GEQGSGRAGG VYTWEEVQSH SSRNDQWLVI DRKVYNITQW
60 70 80 90 100
AKRHPGGIRV ISHCCGEDAT DAFAAFHPDQ RYVRKFMKPL LLGELTPSEP
110 120 130 140 150
SQDHDKNAAL MEDFRDLRKR LESQGLFRTS PLFFILYLGH ILLLEALSVA
160 170 180 190 200
LLWNFGNGWI ISLLLSVLLA TSQAQAGWLQ HDFGHLSVFK NSTWDHLMHK
210 220 230 240 250
FIIGHLKGAS ANWWNHRHFQ HHAKPNIVSK DPDVNMLNIL VLGNILPVEY
260 270 280 290 300
GIKKVKHMPY NHQHRYFFLV GPPLLLPLYF NLHVIQTMYL QRDWVDFAWF
310 320 330 340 350
LSYYARFFMF YSPYYGILGS LVLITFVRFL ESHWFVWVTQ MNHIPMDIDH
360 370 380 390 400
DKHDDWLSMQ LKATCNIEHS QFNDWFSGHL NFQIEHHLFP MMPRHNYSRA
410 420 430 440
SPQVRELCEK YGIQYQVKGL WESWCDIVRS LKKSGELWLD AYLHK
Length:445
Mass (Da):52,133
Last modified:April 29, 2015 - v1
Checksum:iCB34B49B9B356BA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KJ831068 mRNA. Translation: AJQ20793.1.

Similar proteinsi

Entry informationi

Entry nameiFADS2_TACFU
AccessioniPrimary (citable) accession number: A0A0C5PRW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2017
Last sequence update: April 29, 2015
Last modified: November 22, 2017
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families