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Protein

Thymidylate synthase

Gene

thyA

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174PROSITE-ProRule annotation1
Active sitei174NucleophileUniRule annotation1
Binding sitei2025,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei210dUMPUniRule annotation1
Binding sitei2995,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi153 – 154dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi199 – 202dUMPUniRule annotation4
Nucleotide bindingi240 – 242dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:JCM5805K_1958Imported
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)Imported
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000031847 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 299Thymidylat_syntInterPro annotationAdd BLAST271

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.101 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0B8QQD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLNQNSRLC YNERNKKIGR FMTYADKIFK QNIQNILDNG VFSENARPKY
60 70 80 90 100
KDGQTANSKY VTGSFVTYDL QKGEFPITTL RPIPIKSAIK ELMWIYQDQT
110 120 130 140 150
SELAILEEKY GVKYWGEWGI GDGTIGQRYG ATVKKYNIIG KLLDGLAKNP
160 170 180 190 200
WNRRNIINLW QYEDFEETEG LLPCAFQTMF DVRREQDGQI YLDATLIQRS
210 220 230 240 250
NDMLVAHHIN AMQYVALQMM IAKHFSWKVG KFFYFVNNLH IYDNQFEQAN
260 270 280 290 300
ELVKRTASDK EPRLVLNVPD GTNFFDIKPE DFELVDYEPV KPQLKFVLAI
Length:300
Mass (Da):35,063
Last modified:April 1, 2015 - v1
Checksum:iF45AAB8093440AC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BBSI01000030 Genomic DNA Translation: GAM80841.1

Genome annotation databases

EnsemblBacteriaiGAM80841; GAM80841; JCM5805K_1958

Similar proteinsi

Entry informationi

Entry nameiA0A0B8QQD7_LACLL
AccessioniPrimary (citable) accession number: A0A0B8QQD7
Entry historyiIntegrated into UniProtKB/TrEMBL: April 1, 2015
Last sequence update: April 1, 2015
Last modified: February 28, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported