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Protein

Enolase

Gene

eno

Organism
Campylobacter jejuni subsp. jejuni
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (pgm)
  4. Enolase (eno), Enolase (eno), Enolase (eno), Enolase (eno)
  5. Pyruvate kinase (PJ16_01905), Pyruvate kinase (HS58.02), Pyruvate kinase (HS45.02), Pyruvate kinase (HS40.02), Pyruvate kinase (pykF), Pyruvate kinase (CjjRM3196_1416), Pyruvate kinase (HS60.02), Pyruvate kinase (HS42.02), Pyruvate kinase (pyk), Pyruvate kinase (TM42_06765), Pyruvate kinase (HS17.02), Pyruvate kinase (pyk), Pyruvate kinase (HS55.02)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei154SubstrateUniRule annotation1
Binding sitei163SubstrateUniRule annotation1
Active sitei204Proton donorUniRule annotation1
Metal bindingi239MagnesiumUniRule annotation1
Metal bindingi280MagnesiumUniRule annotation1
Binding sitei280SubstrateUniRule annotation1
Metal bindingi307MagnesiumUniRule annotation1
Binding sitei307SubstrateUniRule annotation1
Active sitei332Proton acceptorUniRule annotation1
Binding sitei332Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei383SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotationImported
ORF Names:PJ16_09085Imported
OrganismiCampylobacter jejuni subsp. jejuniImported
Taxonomic identifieri32022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000031887 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation, SecretedUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0B6V6H1
SMRiA0A0B6V6H1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 362Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0B6V6H1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVIEDVRAY EVLDSRGNPT VKAEVTLSDG SVGAAIVPSG ASTGSKEALE
60 70 80 90 100
LRDNDERFGG KGVLKAVANV NETIADEILG LDAFNQTQLD DTLRELDGTN
110 120 130 140 150
NYSNLGANAT LGVSMATARA AAAALGMPLY RYLGGANASI LPVPMCNIIN
160 170 180 190 200
GGAHANNNVD FQEFMIMPFG FTSFKEALRS VCEIYAILKK ELANSGHSTA
210 220 230 240 250
LGDEGGFAPN LANNTEPIDL LMTCIKKAGY ENRVKIALDV ASTEFFKDGK
260 270 280 290 300
YHMEGKAFSS EALIERYVEL CAKYPICSIE DGLAENDFEG WIKLTEKLGN
310 320 330 340 350
KIQLVGDDLF VTNEDILREG IIKKMANAVL IKPNQIGTIT QTMRTVRLAQ
360 370 380 390 400
RNNYKCVMSH RSGESEDAFI ADFAVALNTG QIKTGALARG ERTAKYNRLL
410
EIEFESDEYL GEKL
Length:414
Mass (Da):44,939
Last modified:April 1, 2015 - v1
Checksum:i25C132C6B631FF3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP010301 Genomic DNA Translation: AJK85545.1
RefSeqiWP_002851519.1, NZ_PIAS01000003.1

Genome annotation databases

EnsemblBacteriaiAJK85545; AJK85545; PJ16_09085
PATRICifig|32022.147.peg.1803

Similar proteinsi

Entry informationi

Entry nameiA0A0B6V6H1_CAMJU
AccessioniPrimary (citable) accession number: A0A0B6V6H1
Entry historyiIntegrated into UniProtKB/TrEMBL: April 1, 2015
Last sequence update: April 1, 2015
Last modified: March 28, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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