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Protein

Enolase

Gene

eno

Organism
Thermotoga sp. RQ7
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (TRQ7_03855)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei156SubstrateUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Active sitei206Proton donorUniRule annotation1
Metal bindingi243MagnesiumUniRule annotation1
Metal bindingi286MagnesiumUniRule annotation1
Binding sitei286SubstrateUniRule annotation1
Metal bindingi313MagnesiumUniRule annotation1
Binding sitei313SubstrateUniRule annotation1
Active sitei338Proton acceptorUniRule annotation1
Binding sitei338Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei389SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotationImported
ORF Names:TRQ7_00250Imported
OrganismiThermotoga sp. RQ7Imported
Taxonomic identifieri126738 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000031770 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation, SecretedUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A0B5KU21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 135Enolase_NInterPro annotationAdd BLAST131
Domaini140 – 426Enolase_CInterPro annotationAdd BLAST287

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 368Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

KOiK01689.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR036849. Enolase-like_C.
IPR029017. Enolase-like_N.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0B5KU21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYVEIVDVRA REVLDSRGNP TVEAEVVLED GTVGRAIVPS GASTGKFEAL
60 70 80 90 100
EIRDKDKKRY HGKGVLKAVE NVNETIAPAL IGMNAFDQPL VDKTLIELDG
110 120 130 140 150
TENKSKLGAN AILAVSMAVA RAAANYLGLP MYKYLGGVNA KVLPVPLMNV
160 170 180 190 200
INGGQHADNN LDLQEFMIVP AGFDSFKEAL RAGAEIFHTL KKILHDSGHV
210 220 230 240 250
TAVGDEGGFA PNLSSNEEAI KILIEAIEEA GYKPSEQVFI ALDCAASSFY
260 270 280 290 300
DEEKGVYFVD GEEKSSEVLM GYYEELIAKY PIISIEDPFA EEDWDAFVEF
310 320 330 340 350
TKRVGNKVQI VGDDLYVTNV KRLSKGIELK ATNSILIKLN QIGTVTETLD
360 370 380 390 400
AVELAQKNNM TAIISHRSGE SEDTFIADLA VATNAGFIKT GSLSRSERIA
410 420
KYNQLLRIEE ELGKVAEFRG LSSFYSIKR
Length:429
Mass (Da):46,837
Last modified:April 1, 2015 - v1
Checksum:i51B7552618B0E270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP007633 Genomic DNA. Translation: AJG39905.1.
RefSeqiWP_038068011.1. NZ_CP007633.1.

Genome annotation databases

EnsemblBacteriaiAJG39905; AJG39905; TRQ7_00250.
KEGGithr:TRQ7_00250.

Similar proteinsi

Entry informationi

Entry nameiA0A0B5KU21_9THEM
AccessioniPrimary (citable) accession number: A0A0B5KU21
Entry historyiIntegrated into UniProtKB/TrEMBL: April 1, 2015
Last sequence update: April 1, 2015
Last modified: November 22, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported