Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Biotin synthase

Gene

bioB

Organism
Campylobacter lari NCTC 11845
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi16Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi20Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi23Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi60Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi153Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotationImported
ORF Names:UPTC3659_0178Imported
OrganismiCampylobacter lari NCTC 11845Imported
Taxonomic identifieri1388749 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000031130 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 211Elp3InterPro annotationAdd BLAST206
Domaini183 – 273BATSInterPro annotationAdd BLAST91

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01012.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR034416. BATS_domain_containing.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDG01060. BATS_domain_containing. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0A8HT10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIMLCAISN IASGGCGEDC KYCTQSAHVK TNINKYKRKD IEQIVLEAKM
60 70 80 90 100
AKKNEALGFC LVTAGAGLDD EKLEYVCKVA HAVQKEVEGL LLIACNGIAN
110 120 130 140 150
LEQLKELKKA GIFSYNHNLE TSKEFFPNIC STHTWEQRFQ TNLYAKEAGL
160 170 180 190 200
MLCCGGIYGL GESEADRKSF RASLKELDPF SSPINFFIPN ENLKLKQALL
210 220 230 240 250
DPDEALNIIK DTKKDLPNAH IMVAGGREVV LKERQYEIFN AGASAIVVGD
260 270
YLTTKGEEPS KDIQKLKQMG FSFASSCH
Length:278
Mass (Da):30,771
Last modified:March 4, 2015 - v1
Checksum:iC51BDD5331528035
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP007775 Genomic DNA. Translation: AJD01064.1.
RefSeqiWP_039627323.1. NZ_CP007775.1.

Genome annotation databases

EnsemblBacteriaiAJD01064; AJD01064; UPTC3659_0178.
KEGGicln:UPTC3659_0178.

Similar proteinsi

Entry informationi

Entry nameiA0A0A8HT10_CAMLA
AccessioniPrimary (citable) accession number: A0A0A8HT10
Entry historyiIntegrated into UniProtKB/TrEMBL: March 4, 2015
Last sequence update: March 4, 2015
Last modified: November 22, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported