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Protein

Glutamate 5-kinase

Gene

proB

Organism
Prochlorococcus sp. MIT 0602
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotationSAAS annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7ATPUniRule annotation1
Binding sitei47SubstrateUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei146Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 167ATPUniRule annotation2
Nucleotide bindingi209 – 215ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationSAAS annotationImported, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
ORF Names:EV06_1049Imported
OrganismiProchlorococcus sp. MIT 0602Imported
Taxonomic identifieri1499499 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000030430 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini274 – 343PUAInterPro annotationAdd BLAST70

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR036393. AceGlu_kinase-like_sf.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_sf.
IPR036974. PUA_sf.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A0A2BM52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLWTIKIGT TLLRGTKFFN TKEIINEYAK HISNAKSKGD QIIIVSSGAV
60 70 80 90 100
GLGCNRLGLR NRPNEMNSLQ AAAAVGQGHL ISLYEDAMKN YNYKVAQILL
110 120 130 140 150
TRSDFQSREY FKNASSTIKK LLDWDVIPII NENDSIANEE LKYGDNDTLS
160 170 180 190 200
ALVSTAISAN QLVLLTDIDK LYSEDPKLNK EAKPITDVHN SKELNFLKKN
210 220 230 240 250
SLTSSNWGTG GIKTKLIAAQ IATKNGITVH LADGRQPNCL GEILNGSRGG
260 270 280 290 300
TVFHPSQKPI GTKKSWLAHA LHANGVLKVD EGASNAIQNK GASLLLVGVT
310 320 330 340 350
NVEGNFAANQ AIQIHTSEGI EIAKGISSLS SESIRDKIKN PLKADQSPIV
360
VHRDVLVLSS ELLL
Length:364
Mass (Da):39,475
Last modified:February 4, 2015 - v1
Checksum:iD577739C0211C1AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JNAV01000004 Genomic DNA. Translation: KGG15181.1.
RefSeqiWP_036917527.1. NZ_JNAV01000004.1.

Genome annotation databases

EnsemblBacteriaiKGG15181; KGG15181; EV06_1049.

Similar proteinsi

Entry informationi

Entry nameiA0A0A2BM52_9PROC
AccessioniPrimary (citable) accession number: A0A0A2BM52
Entry historyiIntegrated into UniProtKB/TrEMBL: February 4, 2015
Last sequence update: February 4, 2015
Last modified: November 22, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported