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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Prochlorococcus sp. MIT 0601
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotationSAAS annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14SubstrateUniRule annotation1
Binding sitei72SubstrateUniRule annotation1
Active sitei81PROSITE-ProRule annotation1
Active sitei81Proton donorUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Sitei171Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei202SubstrateUniRule annotation1
Sitei220Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei229Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Lysine biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotationSAAS annotation (EC:5.1.1.7UniRule annotationSAAS annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
ORF Names:EV05_1680Imported
OrganismiProchlorococcus sp. MIT 0601Imported
Taxonomic identifieri1499498 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000030414 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate bindingUniRule annotation2
Regioni220 – 221Substrate bindingUniRule annotation2
Regioni230 – 231Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0A0A2BEG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPINFQKYH GIGNDFIIID GQDSNLPYNI TSPKKDFVKK ITNRNFGIGA
60 70 80 90 100
DGIILILPSN TNNCNVKMKI FNSDGTEPEM CGNGIRCLVK YILDNTPEKL
110 120 130 140 150
LDDFKVETLA GDIPIKLDIN NNIVVDMGVP ILTPPEIPTY LKIGKNGIPQ
160 170 180 190 200
DWLTIGEKSL FVSAISMGNP HAVVEVDQLY DCDINLLGPE LEIHPKFPKH
210 220 230 240 250
TNVHFIKIIR ENYIEALVWE RGCGTTLACG TGACAIVAIL SKLGKCSQNS
260 270 280
VVKLPGGELS IHWPQFGESI YMSGSAEYVY KGQLTIS
Length:287
Mass (Da):31,609
Last modified:February 4, 2015 - v1
Checksum:i0BA7BAA97DF5AC77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JNAU01000005 Genomic DNA Translation: KGG12468.1
RefSeqiWP_036903021.1, NZ_JNAU01000005.1

Genome annotation databases

EnsemblBacteriaiKGG12468; KGG12468; EV05_1680

Similar proteinsi

Entry informationi

Entry nameiA0A0A2BEG5_9PROC
AccessioniPrimary (citable) accession number: A0A0A2BEG5
Entry historyiIntegrated into UniProtKB/TrEMBL: February 4, 2015
Last sequence update: February 4, 2015
Last modified: September 27, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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