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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

Gapdh

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm7293), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839)
  2. Phosphoglycerate kinase 2 (Pgk2), Phosphoglycerate kinase 1 (Pgk1)
  3. no protein annotated in this organism
  4. Gamma-enolase (Eno2), Beta-enolase (Eno3), Alpha-enolase (Eno1), Enolase 4 (Eno4)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei176 – 1761NucleophileUniRule annotation
Sitei203 – 2031Activates thiol group during catalysisUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

NADUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.12UniRule annotation)
Gene namesi
Name:GapdhImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95640. Gapdh.

PTM / Processingi

Proteomic databases

PaxDbiA0A0A0MQF6.

Expressioni

Gene expression databases

ExpressionAtlasiA0A0A0MQF6. baseline.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113213.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 176149Gp_dh_NInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
OMAiKWGEVGA.
OrthoDBiEOG091G0B1Y.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0A0A0MQF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPLPRGPSL GSSGKLRRVF PRPVDKMVKV GVNGFGRIGR LVTRAAICSG
60 70 80 90 100
KVEIVAINDP FIDLNYMVYM FQYDSTHGKF NGTVKAENGK LVINGKPITI
110 120 130 140 150
FQERDPTNIK WGEAGAEYVV ESTGVFTTME KAGAHLKGGA KRVIISAPSA
160 170 180 190 200
DAPMFVMGVN HEKYDNSLKI VSNASCTTNC LAPLAKVIHD NFGIVEGLMT
210 220 230 240 250
TVHAITATQK TVDGPSGKLW RDGRGAAQNI IPASTGAAKA VGKVIPELNG
260 270 280 290 300
KLTGMAFRVP TPNVSVVDLT CRLEKPAKYD DIKKVVKQAS EGPLKGILGY
310 320 330 340 350
TEDQVVSCDF NSNSHSSTFD AGAGIALNDN FVKLISWYDN EYGYSNRVVD

LMAYMASKE
Length:359
Mass (Da):38,653
Last modified:January 7, 2015 - v1
Checksum:iE489231A65BFA67B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC166162 Genomic DNA. No translation available.
RefSeqiNP_001276655.1. NM_001289726.1.
UniGeneiMm.304088.
Mm.309092.
Mm.317779.
Mm.343110.
Mm.392463.
Mm.458138.
Mm.458416.

Genome annotation databases

EnsembliENSMUST00000117757; ENSMUSP00000113942; ENSMUSG00000057666.
GeneIDi14433.
UCSCiuc033iut.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC166162 Genomic DNA. No translation available.
RefSeqiNP_001276655.1. NM_001289726.1.
UniGeneiMm.304088.
Mm.309092.
Mm.317779.
Mm.343110.
Mm.392463.
Mm.458138.
Mm.458416.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113213.

Proteomic databases

PaxDbiA0A0A0MQF6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117757; ENSMUSP00000113942; ENSMUSG00000057666.
GeneIDi14433.
UCSCiuc033iut.1. mouse.

Organism-specific databases

CTDi2597.
MGIiMGI:95640. Gapdh.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
OMAiKWGEVGA.
OrthoDBiEOG091G0B1Y.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

ExpressionAtlasiA0A0A0MQF6. baseline.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA0A0A0MQF6_MOUSE
AccessioniPrimary (citable) accession number: A0A0A0MQF6
Entry historyi
Integrated into UniProtKB/TrEMBL: January 7, 2015
Last sequence update: January 7, 2015
Last modified: September 7, 2016
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.