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Protein

Lipoyl synthase, mitochondrial

Gene

V493_02703

Organism
Pseudogymnoascus sp. VKM F-4281 (FW-2241)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (V493_02703)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi147Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi152Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi158Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi178Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi182Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi185Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:V493_02703Imported
OrganismiPseudogymnoascus sp. VKM F-4281 (FW-2241)Imported
Taxonomic identifieri1420906 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaLeotiomycetesLeotiomycetes incertae sedisPseudeurotiaceaePseudogymnoascus
Proteomesi
  • UP000029327 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 390Elp3InterPro annotationAdd BLAST223

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR031691. LIAS_N.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
PfamiView protein in Pfam
PF16881. LIAS_N. 1 hit.
PF04055. Radical_SAM. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A094B0L7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYAMASSGSR FRCLNAPPLR SAVKAVYNPK TTSAVRGLAT PAGETPIADS
60 70 80 90 100
STTTSTAAAA AAAAAPKGRP SYFKDTPVPT LHEYLDIDQP LTPAEMLALR
110 120 130 140 150
PRTAKVGKKE ITRLPSWLKT SIPSPNTSAG FKAIKADLRG LGLHTVCEEA
160 170 180 190 200
RCPNIGECWG GSDKASATAT IMLMGDTCTR GCRFCSVKTA KKAKGAWDMA
210 220 230 240 250
PLDPHEPENT AEALRRWGLG YVVLTSVDRD DLPDGGSAHF AETIAKIKSK
260 270 280 290 300
SREGGGREIL VEALTGDFAG QLDMVDQVAR SGLDVYAHNV ETVEELTPQV
310 320 330 340 350
RDRRATFAQS LKVLGRAKET VPSLITKTSI MLGLGETEPQ LMDALRRLRE
360 370 380 390 400
VNVDVVTFGQ YMRPTKRHMQ VEEYISPAVF EAWKKRALEM GFLYCAAGPL
410 420 430 440
VRSSYKAGEA FIENVLKQRK GVEAGKVGGE KMAEKVVEVE V
Length:441
Mass (Da):47,662
Last modified:November 26, 2014 - v1
Checksum:iEF2DA3D1A01F1BC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JPJV01001013 Genomic DNA. Translation: KFY28885.1.

Genome annotation databases

EnsemblFungiiKFY28885; KFY28885; V493_02703.

Similar proteinsi

Entry informationi

Entry nameiA0A094B0L7_9PEZI
AccessioniPrimary (citable) accession number: A0A094B0L7
Entry historyiIntegrated into UniProtKB/TrEMBL: November 26, 2014
Last sequence update: November 26, 2014
Last modified: October 25, 2017
This is version 17 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported