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Protein

Thiazole synthase

Gene

thiG

Organism
Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotationSAAS annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotationSAAS annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Schiff-base intermediate with DXPUniRule annotation1
Binding sitei156DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processThiamine biosynthesisUniRule annotationSAAS annotation
LigandSchiff baseUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotationSAAS annotation (EC:2.8.1.10UniRule annotationSAAS annotation)
Gene namesi
Name:thiGUniRule annotation
ORF Names:KP17_19110Imported
OrganismiPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)Imported
Taxonomic identifieri555 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000033091 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 247ThiGInterPro annotationAdd BLAST244

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 183DXP bindingUniRule annotation2
Regioni204 – 205DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR033983. Thiazole_synthase_ThiG.
IPR008867. ThiG.
PANTHERiPTHR34266. PTHR34266. 1 hit.
PfamiView protein in Pfam
PF05690. ThiG. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A093S5H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHIADTTFT SRLLTGTGKF ATPELMLAAL EASGSQLVTM AMKRVDLNGG
60 70 80 90 100
NDAILAPLRQ LGAKLLPNTS GAKTADEAIF AARLAREALG TRWLKLEIHP
110 120 130 140 150
DVKYLLPDPI ETLKAAEQLV KEGFTVLPYC GADPVLCKRL EEVGCAAVMP
160 170 180 190 200
LGAPIGSNQG LQTRDFLRII IEQARIPVIV DAGIGAPSHA ADALEMGADA
210 220 230 240 250
VLVNTAIAVA RDPVAMAQAF RLAVDAGALA RQAGLGSKQF VASATSPLTG
260
FLHQQTEEVE R
Length:261
Mass (Da):27,486
Last modified:November 26, 2014 - v1
Checksum:i526157ABA6260910
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQHN01000033 Genomic DNA. Translation: KFX10345.1.
RefSeqiWP_039487699.1. NZ_JQHN01000033.1.

Genome annotation databases

EnsemblBacteriaiKFX10345; KFX10345; KP17_19110.
PATRICifig|555.20.peg.1761.

Similar proteinsi

Entry informationi

Entry nameiA0A093S5H6_PECCC
AccessioniPrimary (citable) accession number: A0A093S5H6
Entry historyiIntegrated into UniProtKB/TrEMBL: November 26, 2014
Last sequence update: November 26, 2014
Last modified: December 20, 2017
This is version 29 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported