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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

F751_6921

Organism
Auxenochlorella protothecoides (Green microalga) (Chlorella protothecoides)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotationSAAS annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei59S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei135S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei138UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, RNA-binding, Transferase, tRNA-bindingUniRule annotationSAAS annotation
Biological processtRNA processingUniRule annotationSAAS annotation
LigandS-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:F751_6921Imported
OrganismiAuxenochlorella protothecoides (Green microalga) (Chlorella protothecoides)Imported
Taxonomic identifieri3075 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaTrebouxiophyceaeChlorellalesChlorellaceaeAuxenochlorella
Proteomesi
  • UP000028924 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83S-adenosyl-L-methionine bindingUniRule annotation2
Regioni115 – 116S-adenosyl-L-methionine bindingUniRule annotation2
Regioni213 – 215S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK03439.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF16. PTHR23417:SF16. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A087SDZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKKQLYRAR AHSNPLNDQF FDVPLSPKHV NWAEIYPEAY AAAAAAGQPP
60 70 80 90 100
PLVRFADVGC GFGGLTVKLA ENFPGKLVVG MELRDKVTEY VKARVIALRL
110 120 130 140 150
QSPGSYHNAG VVRTNAMKCL PNYFAKGQLE KLFFLFADPH FKAANHRRRI
160 170 180 190 200
IQTTLLAEYA FIMAPGGILY TITDVEELGI WMKEKLDAHP LFEAVPEVEV
210 220 230
ERDPAAALLL QGTEEGQKVA RNGGKTWRAV YRRIQDT
Length:237
Mass (Da):26,465
Last modified:October 29, 2014 - v1
Checksum:i2A955545DF2C6789
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KL662101 Genomic DNA. Translation: KFM23953.1.
RefSeqiXP_011396831.1. XM_011398529.1.

Genome annotation databases

GeneIDi23618312.
KEGGiapro:F751_6921.

Similar proteinsi

Entry informationi

Entry nameiA0A087SDZ9_AUXPR
AccessioniPrimary (citable) accession number: A0A087SDZ9
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: October 25, 2017
This is version 17 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported