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Protein

Thiazole synthase

Gene

thiG

Organism
Pectobacterium carotovorum subsp. brasiliense
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotationSAAS annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotationSAAS annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Schiff-base intermediate with DXPUniRule annotation1
Binding sitei156DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotation
Biological processThiamine biosynthesisUniRule annotationSAAS annotation
LigandSchiff baseUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotationSAAS annotation (EC:2.8.1.10UniRule annotationSAAS annotation)
Gene namesi
Name:thiGUniRule annotation
ORF Names:IW01_12115Imported
OrganismiPectobacterium carotovorum subsp. brasilienseImported
Taxonomic identifieri180957 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000032862 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 247ThiGInterPro annotationAdd BLAST244

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 183DXP bindingUniRule annotation2
Regioni204 – 205DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR033983. Thiazole_synthase_ThiG.
IPR008867. ThiG.
PANTHERiPTHR34266. PTHR34266. 1 hit.
PfamiView protein in Pfam
PF05690. ThiG. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A086ESK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHIADTTFT SRLLTGTGKF ATPELMLAAL EASGSQLVTM AMKRVDLNGG
60 70 80 90 100
NDAILAPLRQ LGIKLLPNTS GAKTADEAIF AARLAREALG TRWLKLEIHP
110 120 130 140 150
DVKYLLPDPI ETLKAAEQLV KEGFTVLPYC GADPVLCKRL EEVGCAAVMP
160 170 180 190 200
LGAPIGSNQG LQTRDFLRII IEQARVPVIV DAGIGAPSHA ADALEMGADA
210 220 230 240 250
VLVNTAIAVA RDPVAMAHAF RLAVDAGGLA RQAGLGSKQF VASATSPLTG
260
FLHQQKEDQQ TEGAVR
Length:266
Mass (Da):28,007
Last modified:October 29, 2014 - v1
Checksum:i244C602FC77ACB28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JPSO01000047 Genomic DNA. Translation: KFF69664.1.
RefSeqiWP_039277359.1. NZ_JPSO01000047.1.

Genome annotation databases

EnsemblBacteriaiKFF69664; KFF69664; IW01_12115.

Similar proteinsi

Entry informationi

Entry nameiA0A086ESK0_PECCA
AccessioniPrimary (citable) accession number: A0A086ESK0
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: December 20, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported