Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Candidatus Vecturithrix granuli
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei562Aminoacyl-adenylateUniRule annotation1
Binding sitei606ATPUniRule annotation1
Metal bindingi900ZincUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi920ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
ORF Names:U27_00149Imported
OrganismiCandidatus Vecturithrix granuliImported
Taxonomic identifieri1499967 [NCBI]
Taxonomic lineageiBacteriaCandidatus Vecturithrix
Proteomesi
  • UP000030661 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 642tRNA-synt_1InterPro annotationAdd BLAST616
Domaini686 – 838Anticodon_1InterPro annotationAdd BLAST153
Domaini897 – 924zf-FPG_IleRSInterPro annotationAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionUniRule annotationAdd BLAST11
Motifi603 – 607"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotationSAAS annotation

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A081C6Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLNLP KTDFPMRANL QKREPELLEK WEQEQIYHQI QAKSAGRPKY
60 70 80 90 100
ILHDGPPYAN GHIHLGTALN KILKDIIVKS KTMMGYDSPY IPGWDCHGLP
110 120 130 140 150
IEHNVEVELT KAGKHLTKSE IRRYCRKYAE KFLDIQRQEF KRLGVFGDWE
160 170 180 190 200
HPYITMDYAY EALTMREFGK FVETASVDKR LKAVRWCISC ETALAEAEVE
210 220 230 240 250
YETDMSPSVY VKFPFSSNPK DIAPELEGKT VFVIIWTTTP WTLPANLAIA
260 270 280 290 300
FHPELPYVAV EVDNSVYIFA EGFLADLVKR FGWTTYRILT TFPGRQLEGF
310 320 330 340 350
ECRHPFIDRA SKLILADYVT LEQGTGCVHT APGHGQEDYE TGLRYGLDIY
360 370 380 390 400
APVDNQGKFT NDVEYFAGMQ VFQANKAIID LMRKDGSLMA SEKVEHQYPH
410 420 430 440 450
CWRCKQPVIF RATAQWFIMM DRNDLRQKAL ESIRHHVRWI PEWGQDRIYS
460 470 480 490 500
MIENRPDWCI SRQRAWGVPI VAFYCNECNT LLLKKDVIDY VADKVAKGGA
510 520 530 540 550
DVWFDLPESE LLPPGTTCEH CGGTTFTKET DILDVWFDSG VSHAAVCEPH
560 570 580 590 600
PDLTWPVDLY LEGSDQHRGW FHSSLLEAIG TRGVAPYRAV LTHGYTVDGD
610 620 630 640 650
GKKMSKSLGN TVAPQDVIKQ YGAEILRLWV SHEDYRTDIT ISDQILQHIA
660 670 680 690 700
DAYRRIRNTC RYILGNLFDF DPEKDMVAYD ELLEIDRWAL HRLQNMIQRV
710 720 730 740 750
RKAYDDFEFH LFFHTFHNFC VVDMSSFYLD ILKDRMYTAK ADSQERRSGQ
760 770 780 790 800
TAMYEILSAM VKLMAPILTF TAEECWGYLA KDGKSVHLQD FPSVNPAWVN
810 820 830 840 850
TGLDERWSQL MDIRGEILKN LEIARKEKVI GHSLDALVEL YASGKTYTLL
860 870 880 890 900
KAFENRLDDI CIVSAVHIFD EGAVLPADAV AAETTEKFYI RIGKAAGEKC
910 920
PRCWHYRTTI GADAAYPDVC AQCAAALR
Length:928
Mass (Da):106,548
Last modified:October 29, 2014 - v1
Checksum:i83BD3608524E7B78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DF820472 Genomic DNA. Translation: GAK60258.1.

Genome annotation databases

EnsemblBacteriaiGAK60258; GAK60258; U27_00149.

Similar proteinsi

Entry informationi

Entry nameiA0A081C6Q3_9BACT
AccessioniPrimary (citable) accession number: A0A081C6Q3
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: March 28, 2018
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported