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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Staphylococcus argenteus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation
ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported, TransferaseImported
Biological processProtein biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR985002:G1H1S-2436-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
ORF Names:A4R31_09435Imported, SAMSHR1132_17440Imported
OrganismiStaphylococcus argenteusImported
Taxonomic identifieri985002 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000187440 Componenti: Unassembled WGS sequence
  • UP000005887 Componenti: Chromosome

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi985002.SAMSHR1132_17440.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini325 – 472GatB_YqeyInterPro annotationAdd BLAST148

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
KOiK02434.
OrthoDBiPOG091H01A7.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB. 1 hit.
InterProiView protein in InterPro
IPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrase-B-like.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
IPR014746. Gln_synth/guanido_kin_cat_dom.
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiView protein in Pfam
PF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
SMARTiView protein in SMART
SM00845. GatB_Yqey. 1 hit.
SUPFAMiSSF55931. SSF55931. 1 hit.
SSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiView protein in PROSITE
PS01234. GATB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A077U816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFETVIGLE VHVELKTDSK MFSPSPAHFG AEPNSNTNVI DLAYPGVLPV
60 70 80 90 100
VNKRAVDWAM RAAMALNMEI ATESKFDRKN YFYPDNPKAY QISQFDQPIG
110 120 130 140 150
ENGYIDIEVD GETKRIGITR LHMEEDAGKS THKGEYSLVD LNRQGTPLIE
160 170 180 190 200
IVSEPDIRSP KEAYAYLEKL RSIIQYTGVS DVKMEEGSLR CDANISLRPY
210 220 230 240 250
GQEKFGTKAE LKNLNSFNFV RKGLEYEEKR QEEELLNGGE IGQETRRFDE
260 270 280 290 300
STGKTILMRV KEGSDDYRYF PEPDIVPLYI DEAWKERVRQ TIPELPDERK
310 320 330 340 350
AKYVNELGLP AYDAHVLTLT KEMSDFFEST IEHGADVKLT SNWLMGGVNE
360 370 380 390 400
YLNKNQVELL DTKLTPENLA GMIKLIEDGT MSSKIAKKVF PELAAKGGNA
410 420 430 440 450
KQIMEDNGLV QISDEATLLK FVNEALDNNE QSVEDYKNGK GKAMGFLVGQ
460 470
IMKASKGQAN PQLVNQLLKQ ELDKR
Length:475
Mass (Da):53,655
Last modified:October 29, 2014 - v1
Checksum:i93397A556F1375E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR821777 Genomic DNA. Translation: CCE59583.1.
LWAP01000036 Genomic DNA. Translation: OMH90004.1.
RefSeqiWP_000545367.1. NZ_LYLU01000022.1.

Genome annotation databases

EnsemblBacteriaiCCE59583; CCE59583; SAMSHR1132_17440.
KEGGisuh:SAMSHR1132_17440.

Similar proteinsi

Entry informationi

Entry nameiA0A077U816_9STAP
AccessioniPrimary (citable) accession number: A0A077U816
Secondary accession number(s): G7ZPT5
Entry historyiIntegrated into UniProtKB/TrEMBL: October 29, 2014
Last sequence update: October 29, 2014
Last modified: February 28, 2018
This is version 30 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported