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Protein

Formamidopyrimidine-DNA glycosylase

Gene

mutM

Organism
Burkholderia pseudomallei (Pseudomonas pseudomallei)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotationSAAS annotation

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAUniRule annotation1
Active sitei3Proton donorUniRule annotation1
Active sitei58Proton donor; for beta-elimination activityUniRule annotation1
Binding sitei94DNAUniRule annotation1
Binding sitei112DNAUniRule annotation1
Binding sitei157DNAUniRule annotation1
Active sitei266Proton donor; for delta-elimination activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, GlycosidaseUniRule annotationSAAS annotationImported, Hydrolase, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation
Biological processDNA damage, DNA repairUniRule annotationSAAS annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)
Short name:
Fapy-DNA glycosylaseUniRule annotation
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutMUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyase MutMUniRule annotation
Gene namesi
Name:mutMUniRule annotationImported
Synonyms:fpgUniRule annotation
ORF Names:BOC42_17370Imported, CXQ84_18740Imported, DP46_1131Imported, DP49_567Imported, ERS012350_00696Imported
OrganismiBurkholderia pseudomallei (Pseudomonas pseudomallei)Imported
Taxonomic identifieri28450 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
Proteomesi
  • UP000029527 Componenti: Unassembled WGS sequence
  • UP000193684 Componenti: Chromosome 1
  • UP000047229 Componenti: Unassembled WGS sequence
  • UP000233327 Componenti: Chromosome 1

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 115FPG_CATInterPro annotationAdd BLAST114
Domaini242 – 276FPG-typeInterPro annotationAdd BLAST35

Sequence similaritiesi

Belongs to the FPG family.UniRule annotationSAAS annotation

Keywords - Domaini

Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105ERD Bacteria
COG0266 LUCA
KOiK10563

Family and domain databases

Gene3Di3.20.190.10, 1 hit
HAMAPiMF_00103 Fapy_DNA_glycosyl, 1 hit
InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR015887 DNA_glyclase_Znf_dom_DNA_BS
IPR020629 Formamido-pyr_DNA_Glyclase
IPR012319 FPG_cat
IPR035937 MutM-like_N-ter
IPR010979 Ribosomal_S13-like_H2TH
IPR000214 Znf_DNA_glyclase/AP_lyase
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF01149 Fapy_DNA_glyco, 1 hit
PF06831 H2TH, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
SMARTiView protein in SMART
SM00898 Fapy_DNA_glyco, 1 hit
SM01232 H2TH, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF81624 SSF81624, 1 hit
TIGRFAMsiTIGR00577 fpg, 1 hit
PROSITEiView protein in PROSITE
PS51068 FPG_CAT, 1 hit
PS01242 ZF_FPG_1, 1 hit
PS51066 ZF_FPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

A0A069AXT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELPEVEVT RRGIEPFVAG RRVERVDVRT AMLRWPVPAG FAEMLRSREV
60 70 80 90 100
LRVERRGKYL LFEVDAGWFI VHLGMTGTLR VLPNDAPPPA PAKHDHVDWI
110 120 130 140 150
FDEFVLRFRD PRRFGAVLWH PRDAGDVHAH PLLASLGVEP FSAAFSGALL
160 170 180 190 200
FGRTRGRTVS VKQALLAGDI VVGVGNIYAS ESLFRAGIRP TTAAGRVSLP
210 220 230 240 250
RYERLADAVR ATLADAIERG GSTLRDFVGS NGESGYFQLD CFVYDRAGEP
260 270
CRVCGAPIRQ IVQGQRSTYF CPNCQR
Length:276
Mass (Da):30,658
Last modified:October 1, 2014 - v1
Checksum:iAA0EE08D77D3C29E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP008755 Genomic DNA Translation: AIO85720.1
CP018380 Genomic DNA Translation: ARK88920.1
CP025264 Genomic DNA Translation: AUG22366.1
CFWD01000001 Genomic DNA Translation: CFL41720.1
JPHA01000293 Genomic DNA Translation: KGD52248.1
RefSeqiWP_004522858.1, NZ_PHRC01000009.1

Genome annotation databases

EnsemblBacteriaiCFT79084; CFT79084; ERS012314_00041
KGD52248; KGD52248; DP49_567
KEGGibut:X994_2168
PATRICifig|1435988.3.peg.5107

Similar proteinsi

Entry informationi

Entry nameiA0A069AXT4_BURPE
AccessioniPrimary (citable) accession number: A0A069AXT4
Entry historyiIntegrated into UniProtKB/TrEMBL: October 1, 2014
Last sequence update: October 1, 2014
Last modified: May 23, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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