UniProtKB - A0A060HD58 (A0A060HD58_XYLFS)
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Protein
Formamidopyrimidine-DNA glycosylase
Gene
mutM
Organism
Xylella fastidiosa MUL0034
Status
Functioni
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotation
Catalytic activityi
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotation
Cofactori
Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | Schiff-base intermediate with DNAUniRule annotation | 1 | |
Active sitei | 3 | Proton donorUniRule annotation | 1 | |
Active sitei | 58 | Proton donor; for beta-elimination activityUniRule annotation | 1 | |
Binding sitei | 92 | DNAUniRule annotation | 1 | |
Binding sitei | 111 | DNAUniRule annotation | 1 | |
Binding sitei | 152 | DNAUniRule annotation | 1 | |
Active sitei | 261 | Proton donor; for delta-elimination activityUniRule annotation | 1 |
GO - Molecular functioni
- class I DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
- class III/IV DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
- damaged DNA binding Source: InterPro
- oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- base-excision repair Source: InterPro
- nucleotide-excision repair Source: InterPro
Keywordsi
Molecular function | DNA-bindingUniRule annotationSAAS annotation, GlycosidaseUniRule annotationSAAS annotation, Hydrolase, LyaseUniRule annotationSAAS annotation, Multifunctional enzymeUniRule annotationSAAS annotation |
Biological process | DNA damage, DNA repairUniRule annotationSAAS annotation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)Short name: Fapy-DNA glycosylaseUniRule annotation Alternative name(s): DNA-(apurinic or apyrimidinic site) lyase MutMUniRule annotation (EC:4.2.99.18UniRule annotation) Short name: AP lyase MutMUniRule annotation |
Gene namesi | Name:mutMUniRule annotation Synonyms:fpgUniRule annotation ORF Names:P303_11215Imported, P303_11670Imported |
Organismi | Xylella fastidiosa MUL0034Imported |
Taxonomic identifieri | 1401256 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella › |
Proteomesi |
|
Interactioni
Subunit structurei
Monomer.UniRule annotationSAAS annotation
Structurei
3D structure databases
ProteinModelPortali | A0A060HD58. |
SMRi | A0A060HD58. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 114 | FPG_CATInterPro annotationAdd BLAST | 113 | |
Domaini | 237 – 271 | FPG-typeInterPro annotationAdd BLAST | 35 |
Sequence similaritiesi
Keywords - Domaini
Zinc-fingerUniRule annotationSAAS annotationPhylogenomic databases
KOi | K10563. |
Family and domain databases
Gene3Di | 3.20.190.10. 1 hit. |
HAMAPi | MF_00103. Fapy_DNA_glycosyl. 1 hit. |
InterProi | View protein in InterPro IPR015886. DNA_glyclase/AP_lyase_DNA-bd. IPR015887. DNA_glyclase_Znf_dom_DNA_BS. IPR020629. Formamido-pyr_DNA_Glyclase. IPR012319. FPG_cat. IPR035937. MutM-like_N-ter. IPR010979. Ribosomal_S13-like_H2TH. IPR000214. Znf_DNA_glyclase/AP_lyase. |
Pfami | View protein in Pfam PF01149. Fapy_DNA_glyco. 1 hit. PF06831. H2TH. 1 hit. |
SMARTi | View protein in SMART SM00898. Fapy_DNA_glyco. 1 hit. SM01232. H2TH. 1 hit. |
SUPFAMi | SSF46946. SSF46946. 1 hit. SSF81624. SSF81624. 1 hit. |
TIGRFAMsi | TIGR00577. fpg. 1 hit. |
PROSITEi | View protein in PROSITE PS51068. FPG_CAT. 1 hit. PS01242. ZF_FPG_1. 1 hit. PS51066. ZF_FPG_2. 1 hit. |
i Sequence
Sequence statusi: Complete.
A0A060HD58-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPELPEVETT LRGLLPYLTN QLIYSLTLRR RTLRWDIPSH IESRLPGHRI
60 70 80 90 100
TTVCRRAKYL LIDTNAGGSL IIHLGMSGTL RLLAPETPLR PHDHVDIMLN
110 120 130 140 150
NRRVLRFNDP RRFGCLLWQE DGQIHPLLQR LGCEPLSDSF NGDYLYQCSR
160 170 180 190 200
ARNVSVKTFL MDQRIVVGVG NIYAAESLFR AGISPLCEAD KISLQRYRRL
210 220 230 240 250
AEVVKDILLY AINRGGTTLR DFLSPDGRPG YFKQELFVYG RQQQPCKQCG
260 270
SLLRQTTIRQ RTTVWCGHCQ G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP006740 Genomic DNA. Translation: AIC13255.1. CP006740 Genomic DNA. Translation: AIC13319.1. |
RefSeqi | WP_004085054.1. NZ_CP006740.1. |
Genome annotation databases
EnsemblBacteriai | AIC13255; AIC13255; P303_11215. AIC13319; AIC13319; P303_11670. |
GeneIDi | 1125693. |
KEGGi | xfl:P303_11215. xfl:P303_11670. |
Similar proteinsi
Entry informationi
Entry namei | A0A060HD58_XYLFS | |
Accessioni | A0A060HD58Primary (citable) accession number: A0A060HD58 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | September 3, 2014 |
Last sequence update: | September 3, 2014 | |
Last modified: | March 28, 2018 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |