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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium tuberculosis
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi249Manganese; via tele nitrogenUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Active sitei273UniRule annotation1
Metal bindingi296ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 277GTPUniRule annotation6
Nucleotide bindingi515 – 518GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotationImported
ORF Names:B7R63_01135Imported, B9D06_05805Imported, B9D92_06840Imported, BVW01_04765Imported, ERS007663_00470Imported, ERS007665_00107Imported, ERS007670_02650Imported, ERS007679_00458Imported, ERS007681_00383Imported, ERS007703_00825Imported, ERS007722_01757Imported, ERS007741_00434Imported, ERS023446_01746Imported, ERS027653_00735Imported, ERS027654_01518Imported, ERS027661_02744Imported, ERS124361_01655Imported, SAMEA2682864_00024Imported, SAMEA2683035_00367Imported
OrganismiMycobacterium tuberculosisImported
Taxonomic identifieri1773 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000196809 Componenti: Unassembled WGS sequence
  • UP000047075 Componenti: Unassembled WGS sequence
  • UP000045842 Componenti: Unassembled WGS sequence
  • UP000041288 Componenti: Unassembled WGS sequence
  • UP000193898 Componenti: Unassembled WGS sequence
  • UP000043293 Componenti: Unassembled WGS sequence
  • UP000049023 Componenti: Unassembled WGS sequence
  • UP000048600 Componenti: Unassembled WGS sequence
  • UP000183655 Componenti: Unassembled WGS sequence
  • UP000197036 Componenti: Chromosome
  • UP000196705 Componenti: Unassembled WGS sequence
  • UP000039953 Componenti: Unassembled WGS sequence
  • UP000182946 Componenti: Unassembled WGS sequence
  • UP000048289 Componenti: Unassembled WGS sequence
  • UP000049258 Componenti: Unassembled WGS sequence
  • UP000038802 Componenti: Unassembled WGS sequence
  • UP000046213 Componenti: Unassembled WGS sequence
  • UP000049114 Componenti: Unassembled WGS sequence
  • UP000045274 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 240PEPCK_NInterPro annotationAdd BLAST219
Domaini245 – 605PEPCK_CInterPro annotationAdd BLAST361

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Family and domain databases

CDDicd00819. PEPCK_GTP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiView protein in InterPro
IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR035077. PEP_carboxykinase_GTP_C.
IPR035078. PEP_carboxykinase_GTP_N.
IPR008210. PEP_carboxykinase_N.
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiView protein in Pfam
PF00821. PEPCK_C. 1 hit.
PF17297. PEPCK_N. 1 hit.
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiView protein in PROSITE
PS00505. PEPCK_GTP. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A045I1R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATIPGLD TAPTNHQGLL SWVEEVAELT QPDRVVFTDG SEEEFQRLCD
60 70 80 90 100
QLVEAGTFIR LNPEKHKNSY LALSDPSDVA RVESRTYICS AKEIDAGPTN
110 120 130 140 150
NWMDPGEMRS IMKDLYRGCM RGRTMYVVPF CMGPLGAEDP KLGVEITDSE
160 170 180 190 200
YVVVSMRTMT RMGKAALEKM GDDGFFVKAL HSVGAPLEPG QKDVAWPCSE
210 220 230 240 250
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM
260 270 280 290 300
LILKLISPEN KAYYFAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM
310 320 330 340 350
RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD
360 370 380 390 400
DGDVWWEGLE GDPQHLIDWK GNDWYFRETE TNAAHPNSRY CTPMSQCPIL
410 420 430 440 450
APEWDDPQGV PISGILFGGR RKTTVPLVTE ARDWQHGVFI GATLGSEQTA
460 470 480 490 500
AAEGKVGNVR RDPMAMLPFL GYNVGDYFQH WINLGKHADE SKLPKVFFVN
510 520 530 540 550
WFRRGDDGRF LWPGFGENSR VLKWIVDRIE HKAGGATTPI GTVPAVEDLD
560 570 580 590 600
LDGLDVDAAD VAAALAVDAD EWRQELPLIE EWLQFVGEKL PTGVKDEFDA

LKERLG
Length:606
Mass (Da):67,253
Last modified:July 9, 2014 - v1
Checksum:iAEE29412E6BCCAE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP022014 Genomic DNA. Translation: ASD90654.1.
CFOE01000025 Genomic DNA. Translation: CFE35811.1.
CNFS01000114 Genomic DNA. Translation: CKR14428.1.
CNFR01000253 Genomic DNA. Translation: CKR49597.1.
CNFU01000619 Genomic DNA. Translation: CKS20673.1.
CHDS01000024 Genomic DNA. Translation: CNG71366.1.
CQGE01000031 Genomic DNA. Translation: CNM97110.1.
CQPW01000009 Genomic DNA. Translation: CNT95664.1.
CQPV01000043 Genomic DNA. Translation: CNU08849.1.
CQPL01000597 Genomic DNA. Translation: CNV18033.1.
CSAD01000034 Genomic DNA. Translation: COU83118.1.
CSAE01000057 Genomic DNA. Translation: COV21073.1.
CHKL01000025 Genomic DNA. Translation: COV68396.1.
CSAM01000108 Genomic DNA. Translation: COW12585.1.
NCTW01000026 Genomic DNA. Translation: OSC67868.1.
NDYU01000012 Genomic DNA. Translation: OWG26845.1.
NDYV01000013 Genomic DNA. Translation: OWG30162.1.
FPTZ01000001 Genomic DNA. Translation: SGH09838.1.
FPXX01000001 Genomic DNA. Translation: SGL33784.1.
RefSeqiWP_003401212.1. NZ_NCKT01000001.1.

Genome annotation databases

EnsemblBacteriaiAMC44504; AMC44504; RN05_0228.
CFE35811; CFE35811; ERS007681_00383.
CKR14428; CKR14428; ERS027653_00735.
CKR49597; CKR49597; ERS027654_01518.
CKS20673; CKS20673; ERS027661_02744.
CNG71366; CNG71366; ERS124361_01655.
CNM97110; CNM97110; ERS023446_01746.
CNT95664; CNT95664; ERS007665_00107.
CNU08849; CNU08849; ERS007663_00470.
CNV18033; CNV18033; ERS007670_02650.
CNY35216; CNY35216; ERS013471_01313.
COU83118; COU83118; ERS007679_00458.
COV21073; COV21073; ERS007703_00825.
COV68396; COV68396; ERS007741_00434.
COW12585; COW12585; ERS007722_01757.
CRH09250; CRH09250; BN1213_00073.
CRH15911; CRH15911; BN1303_03397.
PATRICifig|1773.206.peg.3358.

Similar proteinsi

Entry informationi

Entry nameiA0A045I1R8_MYCTX
AccessioniPrimary (citable) accession number: A0A045I1R8
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: November 22, 2017
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported