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Protein

Isoaspartyl dipeptidase

Gene

BY96_06085

Organism
Escherichia coli O111:NM str. K6722
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolytic cleavage of a subset of L-isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Zinc 1; via tele nitrogen; catalyticUniRule annotation1
Metal bindingi70Zinc 1; via tele nitrogen; catalyticUniRule annotation1
Binding sitei106SubstrateUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Metal bindingi162Zinc 1; via carbamate group; catalyticUniRule annotation1
Metal bindingi162Zinc 2; via carbamate group; catalyticUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Metal bindingi201Zinc 2; via pros nitrogen; catalyticUniRule annotation1
Metal bindingi230Zinc 2; via tele nitrogen; catalyticUniRule annotation1
Binding sitei233SubstrateUniRule annotation1
Active sitei285Proton acceptorUniRule annotation1
Metal bindingi285Zinc 1; catalyticUniRule annotation1
Binding sitei289Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, MetalloproteaseUniRule annotation, Protease
LigandMetal-bindingUniRule annotation, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoaspartyl dipeptidaseUniRule annotation (EC:3.4.19.-UniRule annotation)
Gene namesi
ORF Names:BY96_06085Imported
OrganismiEscherichia coli O111:NM str. K6722Imported
Taxonomic identifieri1446533 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000024307 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0A027U312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 374Amidohydro-relInterPro annotationAdd BLAST111

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 77Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the peptidase M38 family.UniRule annotation

Family and domain databases

CDDicd01308. Isoaspartyl-dipeptidase. 1 hit.
Gene3Di2.30.40.10. 2 hits.
InterProiView protein in InterPro
IPR006680. Amidohydro-rel.
IPR033826. Isoaspartyl-dipeptidase.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR010229. Pept_M38_dipep.
PANTHERiPTHR11647:SF77. PTHR11647:SF77. 1 hit.
PfamiView protein in Pfam
PF01979. Amidohydro_1. 1 hit.
PIRSFiPIRSF001238. IadA. 1 hit.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01975. isoAsp_dipep. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A027U312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDYTAAGFT LLQGAHLYAP EDRGICDVLV ANGKIIAVAS NIPSDIVPDC
60 70 80 90 100
TVVDLSGQIL CPGFIDQHVH LIGGGGEAGP TTRTPEVALS RLTEAGVTSV
110 120 130 140 150
VGLLGTDSIS RHPESLLAKT RALNEEGISA WMLTGAYHVP SRTITGSVEK
160 170 180 190 200
DVAIIDRVIG VKCAISDHRS AAPDVYHLAN MAAESRVGGL LGGKPGVTVF
210 220 230 240 250
HMGDSKKALQ PVYDLLENCD VPISKLLPTH VNRNVPLFEQ ALEFARKGGT
260 270 280 290 300
IDITSSIDEP VAPAEGIARA VQAGIPLARV TLSSDGNGSQ PFFDDEGNLT
310 320 330 340 350
HIGVAGFETL LETVQVLVKD YDFSISDALR PLTSSVAGFL NLTGKGEILP
360 370 380 390
GNDADLLVMT PELRIEQVYA RGKLMVKDGK ACVKGTFETA
Length:390
Mass (Da):41,071
Last modified:July 9, 2014 - v1
Checksum:iF6FDEA5D71E9635D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JHHU01000018 Genomic DNA. Translation: EZD31889.1.
RefSeqiWP_000568417.1. NZ_JHHU01000018.1.

Genome annotation databases

EnsemblBacteriaiEZD31889; EZD31889; BY96_06085.

Similar proteinsi

Entry informationi

Entry nameiA0A027U312_ECOLX
AccessioniPrimary (citable) accession number: A0A027U312
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: June 7, 2017
This is version 18 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported