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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Escherichia coli
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187SubstrateUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Active sitei318UniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Sitei331Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
ORF Names:A8C65_12725Imported, ACU57_22510Imported, AM266_18820Imported, AM408_10950Imported, AML35_10540Imported, AUQ13_03900Imported, BHF46_11765Imported, BUE81_02470Imported, BW690_15770Imported, BZL31_05320Imported, BZL33_16915Imported, CA593_19945Imported, EC3234A_33c01110Imported, EL75_2048Imported, EL79_2087Imported, EL80_2114Imported, ERS085386_01435Imported, JD73_22770Imported, PGD_01626Imported, SK85_01828Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000036012 Componenti: Unassembled WGS sequence
  • UP000051850 Componenti: Unassembled WGS sequence
  • UP000076106 Componenti: Unassembled WGS sequence
  • UP000042887 Componenti: Unassembled WGS sequence
  • UP000028651 Componenti: Unassembled WGS sequence
  • UP000186204 Componenti: Unassembled WGS sequence
  • UP000194908 Componenti: Unassembled WGS sequence
  • UP000030018 Componenti: Unassembled WGS sequence
  • UP000196282 Componenti: Chromosome
  • UP000030000 Componenti: Unassembled WGS sequence
  • UP000050556 Componenti: Unassembled WGS sequence
  • UP000196822 Componenti: Unassembled WGS sequence
  • UP000188724 Componenti: Unassembled WGS sequence
  • UP000029988 Componenti: Unassembled WGS sequence
  • UP000070045 Componenti: Unassembled WGS sequence
  • UP000077380 Componenti: Unassembled WGS sequence
  • UP000194199 Componenti: Chromosome
  • UP000195107 Componenti: Unassembled WGS sequence
  • UP000095493 Componenti: Unassembled WGS sequence

Subcellular locationi

A0A024L3D9:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 342Lyase_1InterPro annotationAdd BLAST331
Domaini408 – 461FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni129 – 132Substrate binding (B site)UniRule annotation4
Regioni139 – 141Substrate bindingUniRule annotation3
Regioni324 – 326Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A0A024L3D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTVRSEKDS MGAIDVPADK LWGAQTQRSL EHFRISTEKM PTSLIHALAL
60 70 80 90 100
TKRAAAKVNE DLGLLSEEKA SAIRQAADEV LAGQHDDEFP LAIWQTGSGT
110 120 130 140 150
QSNMNMNEVL ANRASELLGG VRGMERKVHP NDDVNKSQSS NDVFPTAMHV
160 170 180 190 200
AALLALRKQL IPQLKTLTQT LNEKSRAFAD IVKIGRTHLQ DATPLTLGQE
210 220 230 240 250
ISGWVAMLEH NLKHIEYSLP HVAELALGGT AVGTGLNTHP EYARRVADEL
260 270 280 290 300
AVITCAPFVT APNKFEALAT CDALVQAHGA LKGLAASLMK IANDVRWLAS
310 320 330 340 350
GPRCGIGEIS IPENEPGSSI MPGKVNPTQC EALTMLCCQV MGNDVAINMG
360 370 380 390 400
GASGNFELNV FRPMVIHNFL QSVRLLADGM ESFNKHCAVG IEPNRERINQ
410 420 430 440 450
LLNESLMLVT ALNTHIGYDK AAEIAKKAHK EGLTLKAAAL ALGYLSEAEF
460
DSWVRPEQMV GSMKAGR
Length:467
Mass (Da):50,489
Last modified:July 9, 2014 - v1
Checksum:i3D67E3C0F0FDF40B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP021175 Genomic DNA. Translation: ARR35196.1.
CP021683 Genomic DNA. Translation: ARX14180.1.
HG976952 Genomic DNA. Translation: CDU38545.1.
CYBM01000005 Genomic DNA. Translation: CTT80252.1.
JPQG01000004 Genomic DNA. Translation: KFD74733.1.
JPKH01000020 Genomic DNA. Translation: KGM69889.1.
JPKL01000050 Genomic DNA. Translation: KGM80316.1.
JPMZ01000023 Genomic DNA. Translation: KGM82763.1.
LEUM01000013 Genomic DNA. Translation: KLX97674.1.
LDYI01000145 Genomic DNA. Translation: KPO06780.1.
LIVE01000036 Genomic DNA. Translation: KQJ13304.1.
LOOZ01000008 Genomic DNA. Translation: KXR93459.1.
LOGT01000123 Genomic DNA. Translation: KYS94007.1.
LCVH01000033 Genomic DNA. Translation: OAC44124.1.
MIWY01000009 Genomic DNA. Translation: OEN35164.1.
MSEK01000017 Genomic DNA. Translation: OLR89053.1.
MTKH01000121 Genomic DNA. Translation: ONG27786.1.
MUAC01000034 Genomic DNA. Translation: OUK50519.1.
MUAB01000007 Genomic DNA. Translation: OUK53685.1.
LYBG01000708 Genomic DNA. Translation: OWD69422.1.
RefSeqiWP_001099085.1. NZ_NIDQ01000073.1.

Genome annotation databases

EnsemblBacteriaiCDU38545; CDU38545; PGD_01626.
CTT80252; CTT80252; ERS085386_01435.
KFD74733; KFD74733; JD73_22770.
KGM69889; KGM69889; EL75_2048.
KGM80316; KGM80316; EL80_2114.
KGM82763; KGM82763; EL79_2087.
KLX97674; KLX97674; SK85_01828.
KPO06780; KPO06780; ACU57_22510.
KQJ13304; KQJ13304; AM266_18820.
KXQ39797; KXQ39797; AUQ01_11545.
KYS94007; KYS94007; AML35_10540.
OAC44124; OAC44124; EC3234A_33c01110.
OCO42153; OCO42153; AN669_0219525.
ODH32858; ODH32858; A6803_01945.
OEN35164; OEN35164; BHF46_11765.
PATRICifig|562.10472.peg.1568.

Similar proteinsi

Entry informationi

Entry nameiA0A024L3D9_ECOLX
AccessioniPrimary (citable) accession number: A0A024L3D9
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: October 25, 2017
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported