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Protein

Non-structural protein 1

Gene

NS1

Organism
Influenza A virus (A/blue-winged teal/Wisconsin/548/1979(mixed))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism.UniRule annotationSAAS annotation
Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA.UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-bindingUniRule annotationSAAS annotation
Biological processEukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virusSAAS annotation, Inhibition of host pre-mRNA processing by virusUniRule annotationSAAS annotation, Inhibition of host RIG-I by virusSAAS annotation, Inhibition of host RLR pathway by virus, Interferon antiviral system evasionUniRule annotationSAAS annotation, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1UniRule annotationSAAS annotation
Short name:
NS1UniRule annotation
Gene namesi
Name:NS1Imported
Synonyms:NSUniRule annotation
OrganismiInfluenza A virus (A/blue-winged teal/Wisconsin/548/1979(mixed))Imported
Taxonomic identifieri1445401 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus
Proteomesi
  • UP000119575 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotationSAAS annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotationSAAS annotation, Host nucleusUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer. Interacts with host TRIM25 (via coiled coil); this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated DDX58 CARD ubiquitination. Interacts with human EIF2AK2/PKR, CPSF4, IVNS1ABP and PABPN1.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliA0A023LYM9
SMRiA0A023LYM9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The dsRNA-binding region is required for suppression of RNA silencing.UniRule annotation

Sequence similaritiesi

Belongs to the influenza A viruses NS1 family.UniRule annotationSAAS annotation

Family and domain databases

Gene3Di3.30.420.330, 1 hit
HAMAPiMF_04066 INFV_NS1, 1 hit
InterProiView protein in InterPro
IPR004208 NS1
IPR000256 NS1A
IPR038064 NS1A_effect_dom_like_sp
IPR009068 S15_NS1_RNA-bd
PfamiView protein in Pfam
PF00600 Flu_NS1, 1 hit
SUPFAMiSSF143021 SSF143021, 1 hit
SSF47060 SSF47060, 1 hit

Sequencei

Sequence statusi: Complete.

A0A023LYM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSNTITSFQ VDCYLWHIRK LLSMRDMCDA PFDDRLRRDQ KALKGRGSTL
60 70 80 90 100
GLDLRVATME GKKIVEDILK SETDENLKIA IASSPAPRYI TDMSIEEISR
110 120 130 140 150
EWYMLMPRQK ITGGLMVKMD QAIMDKRITL KANFSVLFDQ LETLVSLRAF
160 170 180 190 200
TDDGAIVAEI SPIPSMPGHS TEDVKNAIGI LIGGLEWNDN SIRASENIQR
210 220 230
FAWGIRDENG GPPLPPKQKR YMARRVESEV
Length:230
Mass (Da):26,049
Last modified:July 9, 2014 - v1
Checksum:iDE875F03C8F08FB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY180295 Viral cRNA Translation: AHN03200.1

Similar proteinsi

Entry informationi

Entry nameiA0A023LYM9_9INFA
AccessioniPrimary (citable) accession number: A0A023LYM9
Entry historyiIntegrated into UniProtKB/TrEMBL: July 9, 2014
Last sequence update: July 9, 2014
Last modified: May 23, 2018
This is version 28 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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