Nucleotide binding

This subsection of the ‘Sequence annotation (Features)’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.

Isolated residues that bind nucleotides are shown as ‘Binding site’.

Example: Q9UJ70

The nature of the nucleotide phosphate which is bound is indicated in the description field, as is any additional information such as the affinity for nucleotide phosphates or uncertainty concerning the precise nucleotide phosphate that is bound.
Examples: P16905, P33164, P23170

When a protein contains several nucleotide phosphate binding sites then they may be numbered.
Example: P16905

We annotate experimentally determined nucleotide binding sites which can be propagated to closely related homologs or within a well-known family (such as serine/threonine protein kinases) using the ‘By similarity’ qualifier.
Examples: Q2M2I8, Q00582

We also annotate predicted ATP-binding sites using the ‘Potential’ qualifier when the presence of such sites is consistent with the function of the protein.
Example: P30621

Related keywords:
For all proteins that bind a nucleotide phosphate: Nucleotide-binding.

Specific keywords related to the identity of the bound nucleotide:
ATP-binding, cAMP-binding, cGMP-binding, FAD, FMN, GTP-binding, NAD, NADP.