Do I still need to use the Sequence Retrieval System (SRS)?

The UniProt search tool allows users to perform Google and SRS-like queries, on UniProtKB, UniRef and UniParc*. Note that it is possible to upload a list of accession numbers and then perform a search in that subset. SRS (e.g. SRS@EBI) is still recommended for users wishing to access other databases

Example: Search for Saccharomyces cerevisiae membrane glycoproteins in UniProtKB

Google-like queries

Using the query builder:

• Select Search in: Protein Knowledgebase (UniProtKB)
• Type Saccharomyces cerevisiae membrane glycoprotein in the Query box
• Click on Search
• Follow the suggestions proposed by the search tool:
• Quote terms "saccharomyces cerevisiae"
• Restrict term "saccharomyces cerevisiae" to organism
• Restrict term "membrane" to keyword
• Restrict term "glycoprotein" to keyword
• Results
• This URL can be bookmarked.

SRS-like queries

Using the query builder:

• Select Search in: Protein Knowledgebase (UniProtKB)
• Click on Fields » to open the query builder
• Select Field: Organism [OS]
• Type saccharomyces cerevisiae (use autocompletion)
• Click on Add & Search
• Click on Fields » to open the query builder
• Select Field: Keyword [KW]
• Type Membrane (use autocompletion)
• Click on Add & Search
• Click on Fields » to open the query builder
• Field: Keyword
• Type Glycoprotein (use autocompletion)
• Click on Add & Search
• Results
• This URL can be bookmarked.

Queries on your personal UniProt dataset

• Click on the Retrieve tab of the toolbar
• Copy/paste your list of accession numbers (i.e. P38903 P47096 P40433 P31787 P28240 P32465 P28319)
• Click on Retrieve
• Click on UniProtKB
• Click on Fields »

  You can now use the UniProt search tool to get a specific subset of entries (i.e. Glycoprotein, Membrane, 3D-structure...) or click on 'Customize display' to choose which columns to show in the results table.