Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q99999 (G3ST1_HUMAN)

Last modified July 22, 2008. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Galactosylceramide sulfotransferase
      Short name(s)=GalCer sulfotransferase
    EC=2.8.2.11
Alternative name(s):
    Cerebroside sulfotransferase
    3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase
    3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase
Gene names
Name: GAL3ST1
Synonyms: CST
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Hide | Top

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes By similarity. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).

Catalytic activity

3'-phosphoadenylyl sulfate + a galactosylceramide = adenosine 3',5'-bisphosphate + a galactosylceramidesulfate.

3'-phosphoadenylyl sulfate + monogalactosylalkylacylglycerol = adenosine 3',5'-bisphosphate + monogalactosylalkylacylglycerol sulfate.

Pathway

Lipid metabolism; sphingolipid metabolism.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane proteinBy similarity.

Tissue specificity

Expressed in kidney proximal tubule, gastric mucosa and adenocarcinoma. Highly expressed in renal cell carcinoma cell lines.

Sequence similarities

Belongs to the galactose-3-O-sulfotransferase family.

Hide | Top

Ontologies

Keywords

   Cellular componentGolgi apparatus
Membrane
   Coding sequence diversityPolymorphism
   DomainSignal-anchor
Transmembrane
   Molecular functionTransferase
   PTMGlycoprotein
   Technical termDirect protein sequencing

Gene Ontology (GO)

   Biological processprotein amino acid N-linked glycosylation Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentintegral to plasma membrane Ref.1

Traceable author statement. Source: ProtInc

membrane fraction Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionsulfotransferase activity Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 423423Galactosylceramide sulfotransferase

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521Signal-anchor for type II membrane protein Potential
Topological domain36 – 423388Lumenal Potential

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation3121N-linked (GlcNAc...) Potential

Natural variations

Natural variant291V → M: dbSNP rs2267161.

Experimental info

Sequence conflict281 – 2822RR → LN AA sequence Ref.1
Sequence conflict4181R → L AA sequence Ref.1
Sequence conflict4221R → E AA sequence Ref.1

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q99999-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 0FD4F2E374447C56

FASTA42348,764
        10         20         30         40         50         60 
MLPPQKKPWE SMAKGLVLGA LFTSFLLLVY SYAVPPLHAG LASTTPEAAA SCSPPALEPE 

        70         80         90        100        110        120 
AVIRANGSAG ECQPRRNIVF LKTHKTASST LLNILFRFGQ KHRLKFAFPN GRNDFDYPTF 

       130        140        150        160        170        180 
FARSLVQDYR PGACFNIICN HMRFHYDEVR GLVPTNAIFI TVLRDPARLF ESSFHYFGPV 

       190        200        210        220        230        240 
VPLTWKLSAG DKLTEFLQDP DRYYDPNGFN AHYLRNLLFF DLGYDNSLDP SSPQVQEHIL 

       250        260        270        280        290        300 
EVERRFHLVL LQEYFDESLV LLKDLLCWEL EDVLYFKLNA RRDSPVPRLS GELYGRATAW 

       310        320        330        340        350        360 
NMLDSHLYRH FNASFWRKVE AFGRERMARE VAALRHANER MRTICIDGGH AVDAAAIQDE 

       370        380        390        400        410        420 
AMQPWQPLGT KSILGYNLKK SIGQRHAQLC RRMLTPEIQY LMDLGANLWV TKLWKFIRDF 


LRW

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase."
Honke K., Tsuda M., Hirahara Y., Ishii A., Makita A., Wada Y.
J. Biol. Chem. 272:4864-4868(1997) [PubMed: 9030544] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-14; 86-101; 102-105; 187-192; 278-286; 372-379 AND 416-423.
Tissue: Renal cell carcinoma.
[2]"Cancer-associated alternative usage of multiple promoters of human GalCer sulfotransferase gene."
Tsuda M., Egashira M., Niikawa N., Wada Y., Honke K.
Eur. J. Biochem. 267:2672-2679(2000) [PubMed: 10785389] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Kidney proximal tubule.
[3]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-29.
Tissue: Kidney.
[5]"Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells."
Honke K., Yamane M., Ishii A., Kobayashi T., Makita A.
J. Biochem. 119:421-427(1996) [PubMed: 8830034] [Abstract]
Cited for: FUNCTION.

Hide | Top

Web resources

GGDB

GlycoGene database

Hide | Top

Cross-references

Sequence databases

D88667 mRNA. Translation: BAA13673.1.
AB029901 Genomic DNA. Translation: BAA89503.1.
CR456350 mRNA. Translation: CAG30236.1.
BC014649 mRNA. Translation: AAH14649.1.
BC019077 mRNA. Translation: AAH19077.1.
RefSeqNP_004852.1.
UniGeneHs.17958

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSG00000128242. Homo sapiens. [Contig view]
GeneID9514.
KEGGhsa:9514.
NMPDRfig|9606.3.peg.21540.

Organism-specific databases

H-InvDBHIX0016374.
HGNCHGNC:24240. GAL3ST1.
HPAHPA001220.
MIM602300. gene.
PharmGKBPA134889661.
GenAtlasSearch...
GeneCardsSearch...
GeneLynxSearch...

Phylogenomic databases

HOGENOMQ99999.
HOVERGENQ99999.

Gene expression databases

ArrayExpressQ99999.
CleanExHS_GAL3ST1.
GermOnlineENSG00000128242. Homo sapiens.

Family and domain databases

InterProIPR009729. Gal-3-0_sulphotransfrase.
[Graphical view]
PANTHERPTHR14647. Gal-3-0_sulfotr. 1 hit.
PfamPF06990. Gal-3-0_sulfotr. 1 hit.
[Graphical view]
ProDomQ99999.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

LinkHubQ99999.
SOURCESearch...
ProtoNetSearch...

Hide | Top

Entry information

Entry nameG3ST1_HUMAN
AccessionPrimary (citable) accession number: Q99999
Secondary accession number(s): Q96C63
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: May 1, 1997
Last modified: July 22, 2008
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents