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Reviewed, UniProtKB/Swiss-Prot Q9VF87 (CYFIP_DROME)

Last modified July 22, 2008. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytoplasmic FMR1-interacting protein
Alternative name(s):
    Specifically Rac1-associated protein 1
      Short name=DSra-1
Gene names
Name: Sra-1
Synonyms: Cyfip
ORF Names: CG4931
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length1291 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Plays a role in guidance and morphology of central and peripheral axons and in synaptic morphology. Also required for formation of cell membrane protrusions and for bristle development.

Subunit structure

Interacts with Fmr1 and Rac1. Component of the WAVE complex composed of Hem/Kette, Scar/Wave and Sra-1/Cyfip where it binds through its C-terminus directly to Hem.

Subcellular location

Cytoplasm. Note= Accumulates in central axons and motor neuron terminals.

Tissue specificity

In the embryo, expressed mainly in the gut and in the developing central nervous system where high levels of expression are found in the CNS neuropile. Expression in the gut diminishes as development proceeds (at protein level). In the adult, expressed specifically in the nervous system.

Developmental stage

Expressed throughout the life cycle with no major peaks of expression. In the embryo, uniquitously and highly expressed at precellular and cellular blastoderm stages.

Miscellaneous

Flies lacking Sra-1 display pupal lethality with defects in axon growth, branching and pathfinding, synaptic length and organization of the neuromuscular junction. They also display defects in cell morphology with cells exhibiting a starfish-like shape with multiple slender cell extensions and loss of actin filaments at the cell periphery.

Sequence similarities

Belongs to the CYFIP family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Nap1Q241501EBI-116862,EBI-603430

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 12911291Cytoplasmic FMR1-interacting protein

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Sequences

Sequence LengthMass (Da)Tools
Q9VF87-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: F3E4254BAD41015B

FASTA1,291149,261
        10         20         30         40         50         60 
MTEKITLADA LSNVEVLDEL SLPDEQPCIE AQPCSIIYKA NFDTNFEDRN GFVTGIAKYI 

        70         80         90        100        110        120 
EEATTHANLN VLLDEGQKHA VMLYTWRCCS RAIPQPKSNE QPNRVEIYEK TVEVLAPEVN 

       130        140        150        160        170        180 
KLLNFMYFQR KAIEAFSGEV KRLCHAEKRK DFVSEAYLLT LGKFINMFAV LDELKNMKSS 

       190        200        210        220        230        240 
VKNDYSTYRR AAQFLKVMSD SHTLQESQNL SMFLATQNKI RDTVKDTLEK IVGYEDLLSD 

       250        260        270        280        290        300 
VVNICVHMFE TKMYLTPEEK HMLVKVMGFG LFLMDSDACN INKLDQKKKI RLDRIDRIFK 

       310        320        330        340        350        360 
NLEVVPLFGD MQIAPFNYIK RSKHFDSSKW PLSSSNAISP QADLMVHLPQ IREDHVKYIS 

       370        380        390        400        410        420 
ELARYTNEVT TTVKENPSDA ENRITADLAL RGLQLLSEWT SVVTELYSWK LLHPTDHHQN 

       430        440        450        460        470        480 
KECPVEAEEY ERATRYNYTS EEKFALIEVI AMIKGLQVLM ARIETVLCEA IRRNIYSELQ 

       490        500        510        520        530        540 
DFVQLSLREP LRKAVKNKKD LIRSIIMSVR ETSADWQKGY EPTDDPVAKG KKDPDGGFRI 

       550        560        570        580        590        600 
QVPRLNVGPS STQLYMVRTM LESLIADKSG GKRTLRKDID GNCLLQIDTF HKTSFYWSYL 

       610        620        630        640        650        660 
LNFSDTLQKC CDLSQLWYRE FYLEMTMGRK VNKCLVRHQH NEECKDLITM EKRIQFPIEM 

       670        680        690        700        710        720 
SMPWILTDHI LQTKEPSMME FVLYPLDLYN DSAYYALTVF RKQFLYDEVE AEVNLCFDQF 

       730        740        750        760        770        780 
VYKLSEQIFA HYKQLAGSIF LDKRFRLECE VLGFNFQSYP RNNRYETLLK QRHVQLLGRS 

       790        800        810        820        830        840 
IDLNKLITQR INANMHKSIE LAISRFEGND ITGIVELEGL LEANRICHKL LSKYLALDNF 

       850        860        870        880        890        900 
DGMVKEANHN VLAPYGRITL HVFVELNYDF LVNYCYNAAT NRFIRTKVNL SSSQAIQREK 

       910        920        930        940        950        960 
PPQMSHYYLW GSKQLNAAYS TQYGQYTGFV GSPHFHAMCR LLGYQGIAVV MDIILKDIVK 

       970        980        990       1000       1010       1020 
PLIQGSLLQF TKTLMIAMPK SCKLPRCEYG SPGVLSYYQA HLTDIVQYPD AKTELFQSFR 

      1030       1040       1050       1060       1070       1080 
EFGNSIIFCL LIEQALSQEE VCDLLHAALF QNIFPRPFCK ENEKPEAKQK RLEAQFANLQ 

      1090       1100       1110       1120       1130       1140 
IVSNVEKIGT AKQAMIAREG DLLTRERLCC GLSIFEVILN RVKSYLDDPV WCGPPPANGI 

      1150       1160       1170       1180       1190       1200 
IHVDECSEFH RLWSALQFVY CIPVRGTEYT IEELFGEGLN WAGCVMIVLL GQQRRFEALD 

      1210       1220       1230       1240       1250       1260 
FCYHILRVQR VDGKDEDVKG IQLKRMVDRI RRFQVLNSQI FSILNKYLKG GDGEGSNVEH 

      1270       1280       1290 
VRCFPPPQHP SVISSSSHYQ DPQKLRQSIN N

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References

« Hide 'large scale' references
[1]"A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P."
Schenck A., Bardoni B., Moro A., Bagni C., Mandel J.-L.
Proc. Natl. Acad. Sci. U.S.A. 98:8844-8849(2001) [PubMed: 11438699] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"DSra-1 is an effector for DRac1 during Drosophila embryogenesis."
Langmann C., Harden N.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[5]Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. expand/collapse author list , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.
Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1152.
Strain: Berkeley.
Tissue: Embryo.
[6]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 156-1291.
Strain: Berkeley.
Tissue: Embryo.
[7]"Abi, Sra1, and Kette control the stability and localization of SCAR/WAVE to regulate the formation of actin-based protrusions."
Kunda P., Craig G., Dominguez V., Baum B.
Curr. Biol. 13:1867-1875(2003) [PubMed: 14588242] [Abstract]
Cited for: FUNCTION, NULL MUTANT.
[8]"CYFIP/Sra-1 controls neuronal connectivity in Drosophila and links the Rac1 GTPase pathway to the fragile X protein."
Schenck A., Bardoni B., Langmann C., Harden N., Mandel J.-L., Giangrande A.
Neuron 38:887-898(2003) [PubMed: 12818175] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FMR1 AND RAC1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, NULL MUTANT.
[9]"WAVE/SCAR, a multifunctional complex coordinating different aspects of neuronal connectivity."
Schenck A., Qurashi A., Carrera P., Bardoni B., Diebold C., Schejter E., Mandel J.-L., Giangrande A.
Dev. Biol. 274:260-270(2004) [PubMed: 15385157] [Abstract]
Cited for: FUNCTION, COMPONENT OF WAVE COMPLEX.
[10]"Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila."
Bogdan S., Grewe O., Strunk M., Mertens A., Klaembt C.
Development 131:3981-3989(2004) [PubMed: 15269173] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HEM, TISSUE SPECIFICITY, NULL MUTANT.

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Cross-references

Sequence databases

AY017343 mRNA. Translation: AAG61254.1.
AY029211 mRNA. Translation: AAK31584.1.
AE014297 Genomic DNA. Translation: AAF55173.1.
BT011115 mRNA. Translation: AAR82782.1.
AY122203 mRNA. Translation: AAM52715.1. Different initiation.
RefSeqNP_650447.1.
UniGeneDm.3506

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ9VF87.

Genome annotation databases

EnsemblCG4931. Drosophila melanogaster. [Contig view]
GeneID41861.
KEGGdme:Dmel_CG4931.
NMPDRfig|7227.3.peg.12961.

Organism-specific databases

FlyBaseFBgn0038320. Sra-1.

Gene expression databases

ArrayExpressQ9VF87.

Family and domain databases

InterProIPR008081. Cytoplasmic_FMR1-int.
IPR016536. Cytoplasmic_FMR1-int_sub.
[Graphical view]
PANTHERPTHR12195. FragX_IP. 1 hit.
PfamPF05994. FragX_IP. 1 hit.
[Graphical view]
PIRSFPIRSF008153. FMR1_interacting. 1 hit.
PRINTSPR01698. CYTOFMRPINTP.
ProDomQ9VF87.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

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Entry information

Entry nameCYFIP_DROME
AccessionPrimary (citable) accession number: Q9VF87
Secondary accession number(s): Q6NP16, Q8MR06
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: May 1, 2000
Last modified: July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents