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Reviewed, UniProtKB/Swiss-Prot Q9VCR6 (SAV_DROME)

Last modified November 25, 2008. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Scaffold protein salvador
Alternative name(s):
    Shar-pei
Gene names
Name: sav
Synonyms: SHRP
ORF Names: CG33193
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Required for cell cycle exit in eye imaginal disk and hid-induced apoptotic cell deaths that are part of normal retinal development. Activation of Ice in eye imaginal disk by either Hid or Rpr is almost completely blocked by Sav expression. Probably part of a same tumor suppression pathway with Sav and Wts.

Subunit structure

Interacts with Wts via its WW domains.

Tissue specificity

Third instar larvae eye disk, expressed in a stripe in the morphogenetic furrow, decreases in the region of the second mitotic wave (SMW) and increases once again posterior to the SMW.

Post-translational modification

Phosphorylated by Hpo.

Sequence similarities

Contains 1 SARAH domain.

Contains 2 WW domains.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

CG15423-RAQ9VQW51EBI-145004,EBI-153446
hpoQ8T0S61EBI-145004,EBI-101858

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608Scaffold protein salvador
PRO_0000076062

Regions

Domain423 – 45634WW 1
Domain458 – 49134WW 2
Domain552 – 59948SARAH
Coiled coil274 – 29421 Potential
Compositional bias107 – 13226His-rich
Compositional bias231 – 2344Poly-Gln
Compositional bias275 – 28915Poly-Gln

Amino acid modifications

Modified residue4131Phosphoserine
Modified residue4161Phosphoserine

Experimental info

Sequence conflict1191S → A in AAL39999. Ref.4
Sequence conflict1941L → M in AAM97280. Ref.1
Sequence conflict2751Missing in AAL39999. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Q9VCR6-1 [UniParc].

Last modified June 1, 2003. Version 2.
Checksum: 05DF47686EE3335E

FASTA60868,146
        10         20         30         40         50         60 
MNYLTILLCN RKPTMLSRRN KEKSQHKEGV VGKYMKKDTP PDISVINVWS DQRAKKKSLQ 

        70         80         90        100        110        120 
RCASTSPSCE FHPRSSSTSR NTYSCTDSQP DYYHARRAQS QMPLQQHSHS HPHSLPHPSH 

       130        140        150        160        170        180 
PHVRSHPPLP PHQFRASSNQ LSQNSSNYVN FEQIERMRRQ QSSPLLQTTS SPAPGAGGFQ 

       190        200        210        220        230        240 
RSYSTTQRQH HPHLGGDSYD ADQGLLSASY ANMLQLPQRP HSPAHYAVPP QQQQHPQIHQ 

       250        260        270        280        290        300 
QHASTPFGST LRFDRAAMSI RERQPRYQPT SSPMQQQQQQ QQQQQQQLQH TQLAAHLGGS 

       310        320        330        340        350        360 
YSSDSYPIYE NPSRVISMRA TQSQRSESPI YSNTTASSAT LAVVPQHHHQ GHLAVPSGSG 

       370        380        390        400        410        420 
GGSLSGSGRG GSSGSVRGAS TSVQSLYVPP RTPPSAVAGA GGSANGSLQK VPSQQSLTEP 

       430        440        450        460        470        480 
EELPLPPGWA TQYTLHGRKY YIDHNAHTTH WNHPLEREGL PVGWRRVVSK MHGTYYENQY 

       490        500        510        520        530        540 
TGQSQRQHPC LTSYYVYTTS AEPPKAIRPE ASLYAPPTHT HNALVPANPY LLEEIPKWLA 

       550        560        570        580        590        600 
VYSEADSSKD HLLQFNMFSL PELEGFDSML VRLFKQELGT IVGFYERYRR ALILEKNRRA 


GQNQNQNQ

« Hide

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References

« Hide 'large scale' references
[1]"Salvador promotes both cell cycle exit and apoptosis in Drosophila and is mutated in human cancer cell lines."
Tapon N., Harvey K.F., Bell D.W., Wahrer D.C.R., Schiripo T.A., Haber D.A., Hariharan I.K.
Cell 110:467-478(2002) [PubMed: 12202036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH WTS.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[5]"Shar-pei mediates cell proliferation arrest during imaginal disc growth in Drosophila."
Kango-Singh M., Nolo R., Tao C., Verstreken P., Hiesinger P.R., Bellen H.J., Halder G.
Development 129:5719-5730(2002) [PubMed: 12421711] [Abstract]
Cited for: FUNCTION.
[6]"The Drosophila Mst ortholog, hippo, restricts growth and cell proliferation and promotes apoptosis."
Harvey K.F., Pfleger C.M., Hariharan I.K.
Cell 114:457-467(2003) [PubMed: 12941274] [Abstract]
Cited for: FUNCTION WITH HPO.
[7]"hippo encodes a Ste-20 family protein kinase that restricts cell proliferation and promotes apoptosis in conjunction with salvador and warts."
Wu S., Huang J., Dong J., Pan D.
Cell 114:445-456(2003) [PubMed: 12941273] [Abstract]
Cited for: PHOSPHORYLATION BY HPO.
[8]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-413 AND SER-416, MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AY131211 mRNA. Translation: AAM97280.1.
AE014297 Genomic DNA. Translation: AAF56090.3.
AY069854 mRNA. Translation: AAL39999.1.
RefSeqNP_788721.1.
UniGeneDm.1289

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:18696N.
IntActQ9VCR6.

Genome annotation databases

EnsemblCG33193. Drosophila melanogaster. [Contig view]
GeneID252554.
KEGGdme:Dmel_CG33193.
NMPDRfig|7227.3.peg.14174.

Organism-specific databases

FlyBaseFBgn0053193. sav.

Phylogenomic databases

HOGENOMQ9VCR6.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-012925-MON.

Gene expression databases

ArrayExpressQ9VCR6.
GermOnlineCG33193. Drosophila melanogaster.

Family and domain databases

InterProIPR011524. SARAH.
IPR001202. WW_Rsp5_WWP.
[Graphical view]
PfamPF00397. WW. 2 hits.
[Graphical view]
SMARTSM00456. WW. 2 hits.
[Graphical view]
PROSITEPS50951. SARAH. 1 hit.
PS01159. WW_DOMAIN_1. False negative.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio843605.

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Entry information

Entry nameSAV_DROME
AccessionPrimary (citable) accession number: Q9VCR6
Secondary accession number(s): Q8MQH6, Q8T9A6, Q9VCR5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: June 1, 2003
Last modified: November 25, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents